The Elucidation of Carcinogenesis in Squamous Cell Carcinoma of the Lung Using Chromate-Induced Lung Cancer of the Rat

铬和烟草烟雾被认为是肺癌发展的一个重要危险因素。为了证实铬酸盐肺癌发生的途径，我们在大鼠近端气道建立了铬酸盐诱发的肺癌模型。我们使用显微镜X射线荧光分析仪（XGT-2700 HORIBA，京都）比较了铬的积累与获得性支气管病变。支气管病变为鳞状细胞癌1/15（7%），原位癌或异型增生7/15（47%），鳞状上皮化生8/15（53%），杯状细胞增生5/15（33%）。所有对侧支气管均正常，显示柱状上皮和纤毛。铬蓄积;正常上皮（n=24）0.500 ± 1.354 S.D.。杯状细胞增生（n=14）0.713±1.062 S.D.。（×1000 cps），鳞状上皮化生（n=8）0.167±0.289 S.D.（×1000 cps），原位癌或异型增生（n=5）5.400±5.320 S.D.（×1000 cps），鳞癌（n=4）1.150 ±1.493 S.D.（×1000 cps）。鳞状细胞癌、癌前病变和不典型增生组织中铬酸盐沉积量高于杯状细胞增生和正常上皮组织。

Chromium as well as tobacco smoke has been known to be an important risk factor in the development of lung cancer. To confirm the pathway in the carcinogenesis of chromate lung cancer, we established chromate induced lung cancer in proximal airway of the rat. And we compared the accumulation of chromium with acquired bronchial lesions using the device, Microscopic X-Ray Fluorescence Analyzer(XGT-2700 HORIBA, Kyoto). The bronchial lesions were squamous cell carcinoma 1/15 (7 %), carcinoma in situ or dysplasia 7/15 (47 %), squamous metaplasia 8/15 (53 %), goblet cell hyperplasia 5/15 (33 %). All contralateral bronchi were normal, showing columnar epithelium with cilia. Accumulation of chromium; normal epithelium (n=24) 0.500 ± 1.354 S.D. (×1000 cps), goblet cell hyperplasia (n=14) 0.713±1.062 S.D. (×1000 cps), squamous metaplasia (n=8) 0.167±0.289 S.D. (×1000 cps), carcinoma in situ or dysplasia (n=5) 5.400±5.320 S.D. (×1000 cps), squamous cell carcinoma (n=4) 1.150 ±1.493 S.D. (×1000 cps). The amount of chromate accumulation in the lesions of SCC, CIS or dysplasia was higher than that of goblet cell hyperplasia and normal epithelium.