Development of simple and accurate assessment method on air pollution with persistent organo-pollutants

2000年7月至2001年11月，我们在日本大坂市的Setsunan大学的Hirakata校区，使用新开发的流量为40 L/min的小容量空气采样器，连续7天采集了2，0个环境空气样品。多氯二苯并呋喃（PCDFs）和共面多氯联苯（Co-PCBs）的分析方法。该方法主要包括加入13 C-内标物，用甲苯或丙酮回流提取，通过多层硅胶柱色谱和氧化铝柱色谱精制，并在高分辨率气相色谱仪上以EI-SIM模式分析二恶英类似物-质谱仪（GC/MS）。因此，首次发现空气中二恶英类似物的污染水平在春至夏季较高，而在冬季较低 ...更多信息 .这一现象与许多研究人员此前报道的许多数据完全相反，这些数据都来自有限的分析数据，例如每年几次。此外，它是明确的，空气污染的每周变化是相当大的水平的最大/最小的比例约为。另一方面，在该实验期间每周收集日本松针样品。空气中二恶英类化合物的气相主要富集在松针中。此外，还明确了其积累水平与松针龄的增加有很好的正相关性，据此，我们根据松针的分析数据，提出了一个简单的二恶英类似物大气污染水平的评价公式。在日本中部大坂府三个地区的应用取得了较好的效果，同时，本课题组与东京环境综合研究所的联合研究也表明，以松针作为空气中二恶英类似物的指示物的生物监测数据与计算机模拟的空气污染结果相吻合，表明生物监测是评价二恶英类大气污染的适宜技术，并揭示了DDT类、HCH类、通过对小容量空气采样器采集的空气样本进行分析，对空气中的三氯甲烷和六氯苯进行了检测，结果表明，这些污染物大多数以气相存在。少

We obtained 2,0 ambient air samples of continuous 7days collecting during a period of July of 2000 to November of 2001 on the campus of Setsunan University, Hirakata, Osaka, Japan, using a newly developed low-volume air sampler with the flow rate of 40 L/min. These air samples were all analyzed for dioxin analogues including polychlorinated dibenzo-p-dioxins (PCDDS), polychlorinated dibenzofurans (PCDFs) and coplanar PCBS (Co-PCBs) using our developed analytical method, which was essentially composing of an addition of 13C-laveled internal standards, extraction with toluene or ace tone under reflux, refined by multi-layer silica gel column chromatography and alumina column chromatography and analysis for dioxin analogues in EI-SIM mode at a high resolution gas chromatograph -mass spectrometer (GC/MS). Consequently, it was first revealed that the air pollution level with dioxin analogues was rather higher during a period of spring to summer seasons and was lower duri ng of winter season … More . This phenomenon was completely contrary to many data previously reported by many researchers, all of which were derived from limited analytical data such as several times per year. In addition, it was made clear that the weekly alteration of air pollution was considerably large with a level of maximum/minimum ratio of ca. 6 throughout this experiment time.On the other hand, Japanese pine needle samples collected weekly during this experimental period. The gas phase of dioxin analogues in the air was confirmed to mainly accumulate in the pine needle. In addition, the accumulation level was also made clearly to have a good positive correlation with an increase of needle age.From these results, we produced a simple assessment formula of air pollution level of dioxin analogues on the basis of the analytical data of pine needle. The method application for three areas i n Osaka Prefecture, central Japan gave a good result.In the meanwhile, a joint research of our group and Environmental General Institute, Tokyo was also made clear that the bio-monitoring data by pine needle as an indicator of dioxin analogues in th e air agreed with the simulation result of air pollution by computer, indicating the bio-nionitoring to insist a suitable technique for assessment of air pollution with dioxin analogues.Furthermore, we revealed the existence state of persistent organo-pollutans including DDT analogues, HCH analogues, chlordane and hexachlorobenzene in the air through the analytical data of air samples collected by the low-volume air sampler, showing the majority of these pollutants to be present as a gas phase. Less