Functional domain identification of transcription factors regulating expression of fungal polysaccharide-degrading enxyme genes.

1. AmyR A.与CGGN_8（C/A）GG的DNA片段结合。两个AmyR分子与共有序列的结合是agdA和taaG 2基因转录激活所必需的。AmyR与酵母Mal激活剂共有5个同源区域（N-末端的Zn和MH 1 -4）。MH 4是AmyR活性信号依赖性调节所必需的，MH 2是转录激活所必需的. X1 nR不仅与GGCTAA结合，而且与含有GGCTGA的序列结合。AoXlnR控制木聚糖分解酶和纤维素分解酶基因的表达。除木聚糖外，纤维素也能诱导X1 nR依赖的转录. Hap复合体A. Hap复合体至少含有HapB、C和E作为亚基。来自每个亚基的核心区域的组装足以用于DNA结合。然而，对于Hap调节基因的反式激活，还需要HapC的N末端区域和HapB的C末端区域。HapC是HapE稳定性所必需的。此外，一种新的因子HapX被鉴定为与Hap复合物相互作用的蛋白质。

1. AmyR A. nidulans AmyR bound to DNA fragments containing CGGN_8(C/A)GG. Binding of two AmyR molecules to the consensus sequence was required for transcriptional activation of the agdA and taaG2 genes. AmyR shares 5 homologous regions with yeast Mal activators (Zn and MH1-4 from N-terminus). MH4 was required for signal-dependent regulation of AmyR activity and MH2 was required for a transcriptional activation.2. X1nR A.oryzae X1nR bound to not only GGCTAA but also GGCTGA containing sequence. AoXlnR controlled expression of both xylanolytic and cellulolytic enzyme genes. In addition to xylan, cellulose could induce X1nR-dependent transcription.3. Hap complex A.oryzae Hap complex at least contains HapB, C, and E as subunits. Assembly of the core regions from each subunit was sufficient for DNA binding. However, for transactivation of Hap-regulated genes, the N-terminal region of HapC and the C-terminal region of HapB were also required. HapC was required for HapE stability. In addition a new factor HapX was identified as a protein which interacted with the Hap complex.