Study on reproductive system of diesel exhaust particles and on prevention of the effects by foods containing flavonoids.

本研究旨在探讨柴油机废气颗粒（DEP）皮下注射对婴儿睾丸和附睾精子的日生精子（DSP）能力、精子活力和畸形的影响。阐明DEP异常的机制，探讨食品预防DEP异常的可能性也是本研究的目的。1)皮下暴露于柴油废气颗粒（DEP）最低剂量（0.22mg /只和0.074mg /只）的小鼠出生阴道闭锁畸形儿。2)我们还研究了DEP对睾丸和附睾每日精子生成量、精子活力和精子畸形的剂量依赖性。用倒u型toDEPdose观察其作用。3)许多文献认为芳烃受体（AhR）可能与畸形的发生有关。因此，我们采用不同AhR活性的4种小鼠，研究了AhR活性的差异与睾丸和附睾每日精子产生率、精子活力和精子形成之间的关系。4)睾丸中AhR活性与每日精子产生能力呈负相关，精子活力与AhR活性呈正相关。5)此外，在4种小鼠中。结果表明，在4种小鼠中，AhR与AhR活性有关。这些结果提示，AhR活性可能对精子的产生、活力和精子形态异常起重要作用。6)在dep依赖的体外AhR刺激系统中，考察了食品中黄酮类化合物对AhR的抑制能力。抑制能力依次为：核糖素>、mirytin >、resubatorol。洋葱和其他蔬菜中含有大量的类黄酮。

The aims of this study are to examine the changes of daily sperm production (DSP) ability, viability of sperm and malformation of infants and sperm in testis and epididymis by diesel exhaust particles (DEP) administrated subcutaneously. And, to clarify the mechanism of the abnormality by DEP and to examine the possibility for preventing the abnormality by foods were also the aims of this study.1)We found that malformation infants with vaginal atresia were born in mouse exposed subcutaneously to diesel exhaust particles (DEP) at the lowest dose of DEP (0.22mg and 0.074mg per mouse).2)we examined also dose dependency of DEP on daily sperm production, viability of sperm and malformation of sperm in testis and epididymis. Their effects were observed with upside-down U-type toDEPdose.3)Many papers suggest that arylhydrocarbon receptor (AhR) may associate with the occurrence of malformations. Therefore, we examined the relationship whether the difference of AhR activity would associate with the ratio of daily sperm production, viability of sperm and formation of sperm in testis and epididymis by using 4 species mice with different AhR activity.4)we could find the reverse relationship between AhR activity and daily sperm production ability and the positive relationship between sperm viability and AhR activity in testis.5)Furthermore, within 4 species of mice. From these results, it was suggested that AhR related with AhR activity within 4 species of mice. From these results, it was suggested that AhR activity may play an important role on sperm production, viability and the morphological abnormality of sperm.6)AhR-inhibiting (Ah-I) ability by flavonoids in foods was also examined in DEP-dependent AhR stimulation in vitro system. The inhibitory ability was ordered askerucetin> mirystin> resuberatorol. These flavonoids are contained very much in onion and other vegetables.