Multinuclear giant cell formation mechanism of Krabbe disease patient brain

这个项目的目标是了解精神病素形成多核细胞的机制。3年来的研究结果表明：(1)精神病素抑制胞质分裂，并通过破坏细胞膜运输至卵裂沟而诱导多核细胞；(2)精神病毒所致细胞内细胞器分布异常。精神病毒素诱导溶酶体和EARL内吞体选择性分散而不影响细胞的内吞活性。(3)酵母系统分离与抗精神病毒素有关的基因。其中一个基因编码色氨酸渗透酶。色氨酸通过高尔基体-液泡膜运输系统降解色氨酸渗透酶。

The goal of this project is to understand the mechanism underlining multinuclear cell formation by psychosine. The following findings were obtained during 3years.(1) Multinuclear giant cell formation mechanism by PsychosinePsychosine inhibited cytokinesis and induced multinuclear cells by disrupting membrane transport to the cleavage furrow during cytokinesis.(2) Abnormal distribution of intracellular organelles by psychosine.Psychosine induced selective dispersal of lysosomes and earl endosomes without affecting the endocytosis activity of the cells.(3) Genes involved in resistance to psychosine in yeastPsychosine resistant genes were isolated using yeast system. One of genes encodes tryptophan permease. Tryptophan permease was degraded by the treatment of psychosine via the Golgi-vacuole membrane transport system.