Practical use of cloning technology for NOD and dy disease model mice production

本研究的目的是利用胚胎干细胞建立胚胎克隆系统，为疾病及其治疗的基础研究做出贡献。在此，我们集中于用作I型糖尿病模型的NOD非肥胖糖尿病小鼠，以及用作肌营养不良的dy和mdx小鼠，并尝试从源自上述疾病模型小鼠的ES细胞产生克隆小鼠。所得结果总结如下。1）通过标准核移植，使用从NOD小鼠建立的ES细胞成功地产生克隆囊胚，但迄今为止没有获得幼仔。2）从dy和mdx小鼠中建立了可传代培养的ES细胞。3)使用这些ES细胞作为供体细胞核，产生了克隆囊胚，但同样没有获得幼仔。我们一起从NOD、dy和mdx小鼠中建立了ES细胞，但未能产生克隆个体。进一步的研究可能是必要的，以澄清这里建立的ES细胞的发育能力。

The present study was conducted to develop embryo cloning systems using ES cell and contribute to fundamental studies for disease and its treatments. Here, we focused on NOD non-obese diabetic mouse used as a model for diabetes typeI, and dy and mdx mice used as muscular dystrophy and tried to produce cloned mice from ES cells derived from above disease model mice. The results obtained were summarized as follows. 1)By standard nuclear transfer, cloned blastocysts were successfully produced using ES cells established from NOD mice, but no pup was obtained to date. 2)ES cells, which can be cultured over several passages, have been established from dy and mdx mice. 3)Using these ES cells as donor nuclei, cloned blastocysts were produced, but again no pup was obtained. Together, we have established ES cells from NOD, dy and mdx mice, but failed to produce cloned individuals. Further studies may be necessary to clarify developmental competence of ES cells established here.