The epidemiological study on human adverse health effects 30 years after sprayed dioxins polluted environment in Vietnam

为研究人类样本（包括母乳、脂肪组织和血清）中二恶英的含量，以及在喷洒二恶英污染越南环境后30年，这些样本对人体健康的影响，在2002年至2004年期间，在越南中部两条村庄的越南居民中进行了四次检测。二恶英接触区位于广治省Cam Chinh公社，在那里喷洒了除草剂（Quanate Quang Tri至QT），而河静省Cam Chinc公社（Quanate Ha Tinh至HT）被选为对照区，在那里战争期间没有喷洒除草剂。这两个公社被北纬17度非军事区（越南战争时的军事分界线）隔开，用高分辨率质谱仪进行定量（人力资源管理系统; JEOL MStation-JMS 700），配备气相色谱仪（HP-6980）。脂肪组织和血清中的人在除草剂暴露区在越南显着高于二恶英未暴露的对照区的人。然而，在大多数样品中，TCDD贡献不到总I-TEQ的20%。这一结果与以往报道的橙子剂中二恶英主要以TCDD为主的观点不同。1，2，3，7，8-PCDD和2，3，4，7，8-PCDF是本研究中毒性当量的主要来源，有必要对其来源进行研究。母亲体内PCDD/Fs总量与胆碱酯酶呈显著负相关。调整婴儿年龄后，母乳中二恶英浓度与婴儿胸围有显着相关性，尽管我们的结果没有显示两个地区婴儿的生长差异。

To examine dioxins levels in human specimen including breast milk, adipose tissue and serum and their relation with human adverse health effects over 30 years after sprayed dioxins polluted Vietnamese environment, Vietnamese inhabitants of two villages in central part of Vietnam were selected in four examinations between 2002 and 2004. Dioxin-exposed area is located in Cam Chinh commune in Quang Tri province where herbicides were sprayed (abbreviate Quang Tri to QT), while Cam Phuc commune in Ha Tinh province (abbreviate Ha Tinh to HT) is selected as control area where herbicides were not sprayed during the War. These two communes were separated by the demilitarized zone of latitude 17 degrees north line that was a military boundary in Vietnam War.Quantitation was performed by a high resolution mass spectrometer (HRMS ; JEOL MStation-JMS700) equipped with a gas chromatograph (HP-6980).Dioxin levels of human samples including breast milk, adipose tissue and serum in people in herbicide-exposed area in Vietnam is significantly higher than those in people in dioxin-unexposed control area. However, TCDD contributed less than 20% of the total I-TEQ in most samples. These results were different from the former reports that TCDD was a useful indicator for toxicity of Agent Orange since TCDD occupied most of dioxins. It is necessary to investigate the origin of 1,2,3,7,8-PCDD and 2,3,4,7,8-PCDF which were the most contributor in the total TEQ in this study.Biochemical indicators in sera such as liver or thyroid functions were not significantly different between two groups. Significant negative correlations were showed between Total PCDD/Fs and cholinesterase of mothers. Dioxin concentration in breast milk has significant association with chest measurement of infants after adjustment of age of infants although our results did not show difference between the growths of infants in two areas.