BIOCATALYSTS MINED FROM CYTOCHROME P450 LIBRARIES: AN INNOVATIVE TOOL FOR ACCELERATING DRUG DEVELOPMENT

细胞色素p4500s （p4500s）是一个酶家族，可能是已知的最通用的生物催化剂。DNA洗牌是一种新兴的技术，它采用编码酶家族的基因，并创建具有改进和新特性的催化剂库。我们将获得概念证明，即洗牌后的P450文库可以被筛选和优化，用于药物开发中的生物催化剂。这里开发的方法将克服当前药物开发的两个关键瓶颈：新候选药物的优化和代谢分析。这将在加速正在开发的药物的优化和安全性测试方面产生重要的好处。

The cytochrome P450s (P450s) are a family of enzymes that are perhaps the most versatile biological catalysts known. DNA shuffling is an emerging technique that takes the genes encoding families of enzymes and creates libraries of catalysts with both improved and novel properties. We will obtain proof of concept that shuffled P450 libraries can be screened and optimized for use as biocatalysts in drug development. The methodologies developed here will overcome two critical bottlenecks in current drug development: the optimisation and metabolic profiling of new drug candidates. This will yield important benefits in accelerating the optimisation and safety testing of drugs under development.