Production of usable materials from sugar beet pulp using bioreactor with equipment for electrical pre-treatment of substrate.
Production of usable materials from sugar beet pulp using bioreactor with equipment for electrical pre-treatment of substrate.
批准号:
15560712
负责人:
TOKUDA Hiroharu
金额:
$1.28万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2003
资助国家:
日本
项目状态:
已结题
起止时间:
2003 至 2005
中文摘要
1.甜菜果肉高水解活性真菌的筛选。从全国各地采集的土壤中筛选出了对甜菜果肉具有较高水解活性的真菌。这种细菌可以水解甜菜果肉,并产生与阿拉伯糖相关的糖。经形态学观察和要求分析,确定该菌株为烟曲霉。甜菜果肉酶解的电预处理。用30 V直流电流对甜菜果肉进行预处理,并对其进行水解。通过预处理提高了甜菜果肉的水解程度,发现直流电(1)预处理导致甜菜果肉膨胀和雾化;(2)提高了甜菜粕中半纤维素的提取率,使潜在阿拉伯糖的提取率达到80%左右。真菌固定化及其甜菜果肉水解特性研究。以人造丝无纺布为支撑材料,采用气相培养法固定化了具有高水解活性的烟曲霉和/或黑曲霉。最佳水解温度为40℃,水解率为70%。从甜菜浆中生产可用材料。通过电处理从甜菜纸浆中提取可用物质。通过电处理,从100g甜菜浆中洗脱出约28g可溶性物质。其成分为阿拉伯糖(约30%)、木糖(约9%)、果胶(约9%)、半乳糖醛酸(约3%)和其他(约49%)。4-2从甜菜果肉水解物中生产抗菌物质。对甜菜果肉水解液进行浓缩,并用酸化Pediococcus进行发酵。培养液中乳酸含量为14g/l,乙酸含量为9g/l,对植物病原菌Erwiniw carotovora sub.sp.Carotovoa具有一定的抑菌活性。
英文摘要
1.Screening of fungi having high hydrolysis activity of beet pulp.Fungus having high hydrolysis activity of beet pulp was screened from the soils collected from many parts of the nation. The bacterium hydrolyzed the beet pulp, and it produced arabinose related sugar. As a result of morphological observation and request analysis, it became clear that this strain was Aspergillus fumigatus.2.Electrical pre-treatment of beet pulp for its enzymatic hydrolysis.Beet pulp was pre-treated with 30 V of direct electric current and was hydrolyzed. The hydrolysis degree of beet pulp was increased by pre-treatment and it was found that the pre-treatment with direct electric current (1)causes swelling and atomization of beet pulp ; and (2)enhances the elution of hemicellulose from beet pulp, resulting in about the ability to extract 80% of the potential arabinose.3.Immobilization of fungi and its characteristic of beet pulp hydrolysis.The immobilization of Aspergillus fumigatus and/or Aspergillus niger having high hydrolysis activity of beet pulp was done by gas phase cultivation method using non-woven fabric made of rayon as a support material. The optimal temperature and the rate of hydrolysis was 40℃ and 70%, respectively.4.Production of usable materials from beet pulp.4-1 The extraction of usable materials from the beet pulp by electrical treatment.By the electrical treatment, about 28g of soluble materials were eluted from 100g of beet pulp. The components of them were arabinose(about 30%), xylose(about 9%), pectin(about 9%), galacturonic acids(about 3%) and others(49%).4-2 Production of antibacterial substance from beet pulp hydrolyzate.Beet pulp hydrolyzate was concentrated, and was fermented by Pediococcus acidilactici. The culture broth contained 14g/l of lactic acid and 9g/l of acetic acid, and had antibacterial activity against Erwiniw carotovora sub.sp.Carotovoa which was one of the plant pathogenic microbe.
期刊论文(6)
专著(0)
科研奖励(0)
会议论文
各種農産廃棄物の加水分解におよぼす電解前処理効果
电解预处理对各类农业废弃物水解的影响
DOI:
--
发表时间:
期刊:
日本醸造学会誌 (投稿中)
影响因子:
--
作者:
[徳田, 田中, 本間, 中西]
通讯作者:
中西
Effects of electrical pretreatment on the hydrolysis of agricultural wastes.(in Japanese)
电预处理对农业废弃物水解的影响(日文)
DOI:
--
发表时间:
期刊:
Journal of Brewing Society of Japan (Under contribution)
影响因子:
--
作者:
[H.Tokuda, M.Tanaka, H.Homma, K.Nakanishi]
通讯作者:
K.Nakanishi
セルロース系バイオマスからの物質生産方法および物質生産システム
纤维素生物质的材料生产方法和材料生产系统
DOI:
--
发表时间:
2003
期刊:
影响因子:
--
作者:
[]
通讯作者: