Reproductive and population ecology of wild Arabidopsis

a.濒临灭绝的堪察加拟南芥的当前分布。被调查了。对过去记录中所有可能的地点进行了调查，以确定目标植物的当前发生情况。在滋贺县、三重县、富山县和高知县分别有3个、1个、1个和1个种群。该物种在爱爱县和爱知县可能已经灭绝。b.以最大种群Biwako种群为研究对象，对其种群空间分布格局进行了调查。kawasakiana。通过对自然种群进行物候记录，确定萌发期和花期。c.通过去雄和套袋处理等一系列田间试验，揭示了甘桦的繁殖体系。kawasakiana。结果表明，该物种主要通过自交授粉产生种子。本种具有自花授粉机制。我们使用生长箱进行生长实验（其中一个是由该基金购买的，Koitotoron HNM-S）。我们控制温度和光周期，并设计实验来鉴定遗传变异。结果表明，南方种群对春化开花的要求比北方种群强。我们在所有研究群体中都发现了显著的遗传变异。我们在一个拟南芥（Arabidopsis halleri）姐妹群体中发现了毛状体存在的多态性。在兵库县中部的一个地方。有毛和无毛的个体比例为1:1。我们在没有毛状体的个体中发现了GL1（毛状体丢失的候选基因）序列中的突变位点。

a.Current distributions of endangered Arabidopsis kamchatca ssp. kawasakiana were investigated. All possible localities from the past records were investigated for the current occurrences of the target plant. We confirmed existences of 3,1,1, and 1 populations in Shiga, Mie, Toyama, and Kochi Prefectures, respectively. The species probably has extinct in Ehime and Aichi Prefectures.b.Spatial distribution patterns of individuals were investigated at Biwako population, the largest population of A. kamchatica ssp. kawasakiana. We recorded phenology and periods for germination and flowering in th natural population were determined.c.We conducted a set o field experiments including emasculation and bagging treatments to reveal breeding systems of A. kamchatica ssp. kawasakiana. It turned out that the species produces seeds by primarily by self polination. The species has a mechanisms of auto-polination.d.We conducted growth experiments using growth chambers (one of them is purchased by this grant, Koitotoron HNM-S). We controlled temperatures and photoperiods, and the experiments were designed to qualify genetic variation. Results indicated that southern populations have stronger requirements of vernalization for flowering compared with those of northern populations. We detected significant genetic variation for all of the studied populations.e.We discovered polymorphism on trichome presence in a population of Arabidopsis halleri sibsp. gemmifera in a central Hyogo. The frequencies of individuals with and without trichome was 1:1. We found mutation sites within a sequence of GL1, candidate gene for trichome loss, for the individuals without trichome.