Molecular Biological Study of Species Specific Carcinogenicity of Food Red No.2 (amaranth)

有关食物红2的物种特异性致癌性的资料如下：1。食物红2对小鼠结肠粘膜DNA损伤的影响不明显。在给药前应用胆汁结扎术，结肠DNA损伤消失。在食物红色到达的地方观察到结肠中的DNA损伤。从大鼠或小鼠分离的结肠条与口服食物红2的小鼠结肠内容物孵育后，均显示DNA损伤。从这些结果来看，大鼠和小鼠在结肠DNA损伤方面的差异可能是由于两种物种之间食物红代谢的差异。我们用高效液相色谱法鉴定了口服食物红2后小鼠结肠含量的一个特定峰。多次给药5天后，小鼠结肠DNA损伤消失，可能是结肠粘膜细胞敏感性降低所致。为了更准确地向人类推断食物红2致癌性的啮齿动物数据，有必要鉴定食物红2在大鼠中的代谢物。

The data concerning to the species specific carcinogenicity of food red 2 were as follows :1.DNA damage in colon mucous membrane of mice induced by food red 2 by ip injection was less prominent than po administration.2.That DNA damage in colon disappeared when the bile legation was applied before po administration.3.That DNA damage in colon was observed where the food red 2 reached.4.Colon strips Isolated from either rats or mice showed DNA damage, when incubated with colon content of mice after oral administration of food red 2.From these results, the difference between rats and mice in DNA damage of colon is suggested to be due to the difference in food red 2 metabolism between the two species.Using HPLC we identified a specific peak in the colon content of mice after oral administration of food red 2.After the 5 days multiple administration of food red 2, colon DNA damage in mice disappeared, which may be the decrease of sensitivity of cells in colon mucous membrane.In order to more precisely extrapolate rodent data to humans concerning the carcinogenicity of food red 2, identification of food red 2 metabolite in rats would be necessarily.