Separation and resolution of bioactive compounds, such as drug, utilizing hair roots as a specimen

众所周知，许多化合物在头发中积累。因此，毛发分析提供了一种确定个人长期化学品暴露史的重要手段。尽管有许多关于毛发分析的论文，但对药物在发根中分布的研究非常罕见，因为人们认为药物从循环血液渗透到毛发中非常缓慢。然而，最近的研究表明，所施用的药物通过发根相对迅速地并入毛干中。结果表明，药物监测的可能性，在头发根后不久给药，类似于药物监测使用blood.In目前的研究中，生物活性化合物，如药物和生物胺的测定与电喷雾电离质谱（ESI-MS）的HPLC耦合。将所提出的方法应用于腹膜内（i. p.）苯并二氮杂类药物的给药 ...更多信息 （三唑仑、阿普唑仑、艾司唑仑和咪达唑仑）。在毛干中鉴定出伴随母体药物的各种代谢产物。苯二氮卓类药物单次腹腔内给药后，还对发根和血浆中的母体药物和代谢产物进行了时程研究。结果表明，头发样品中母体药物和代谢产物的浓度主要取决于血浆中的浓度，因此，为了阐明药物在毛发中的分布和解释回顾性药物暴露，研究药物在毛发中的归宿并与血浆中的归宿进行比较似乎是重要的。用柱切换HPLC-ESI-MS法测定头发中HA的含量。该方法已用于黑曲霉大鼠和健康志愿者头发中HA的测定。用该方法测定了大鼠和人血浆中的HA。由于该方法提供了良好的精密度和痕量检测头发中的HA，分析技术似乎是适用于测定头发中的各种生物胺。少

It is well known that many compounds are accumulated in hair. Thus, the hair analysis provides one important means for determining individual past history of long-term chemical exposure. In spite of many papers regarding hair analysis, the studies of drug disposition in the hair root are very rare, because it is believed that the permeation of drugs into the hair from circulated blood is very slow. However, recent research has shown that administered drugs are relatively promptly incorporated into the hair shaft via the hair roots. The results suggest the possibility of drug monitoring in hair roots shortly after drug administration, similar to drug monitoring using blood.In the present studies, bioactive compounds such as drugs and biogenic amines were determined by HPLC coupled with electrospray ionization-mass spectrometry (ESI-MS). The proposed procedure was applied to the determination in hair shaft of Dark Agouti rats after intraperitoneal (i.p.) administration of benzodiazepines … More (triazolam, alprazolam, estazolam and midazolam). Various metabolites accompanied with parent drugs were identified in the hair shaft. The time course studies of parent drugs and the metabolites in both hair root and plasma were also carried out after single i.p. administration of benzodiazepines. The results suggested that the concentrations of parent drugs and the metabolites in the hair samples were mainly dependent upon those in the plasma.Consequently, investigation of the fate of a drug in the hair roots and a comparison with that in the plasma seem to be important in order to elucidate drug disposition in hair and interpret the retrospective drug exposure.Sensitive determination of histamine (HA) in hair was also carried out by column-switching HPLC-ESI-MS. HA was labeled with excess amounts of DBD-F at 60℃ for 30 min in a basic medium. The proposed method was applied to HA determination in the hair of Dark Agouti rats and healthy volunteers. The determination of HA in the plasma of rats and humans was also successfully performed by this method. Because the proposed method provides good precision and trace detection of HA in hair, the analytical technique seems to be applicable for the determination of various biogenic amines in hair. Less