Establishment of the monitoring method in anti-platehet therapy using with modified collagen bead column.

血小板在维持正常血流止血中起着至关重要的作用，该系统受到干扰可导致病理性血栓形成和血管闭塞，从而导致中风、不稳定心绞痛和心肌梗死等血管意外。为了防止这种病理性血栓的形成，抗血小板药物被开发出来，目前广泛用于血管疾病的预防和治疗。在众多评价血小板功能和抗血小板药物对血小板功能影响的方法中，血小板粘附和聚集试验是最常用的方法。它们在临床环境中得到了有限的批准，因为它们有几个缺点，包括检测血小板弱活化的相对不敏感，缺乏可重复性或临床相关性，或测量程序繁琐。为了克服这些缺点，我们开发了一种含有小尺寸胶原蛋白珠的新柱。小型胶原蛋白珠柱由球形共聚物-塑料珠包被猪I型胶原蛋白，装入聚氯乙烯管。血液通过小尺寸的胶原珠柱。收集经柱前后全血，计数血小板数目。血小板保留率（%）计算为100 ×[（传代前血小板计数）-（传代后血小板计数）]/（传代前血小板计数）。除阿司匹林外，ADP清道夫和ADP受体阻滞剂似乎支持该系统中的血小板活化。口服噻氯匹定的健康志愿者的血液样本显示“ADP柱”中的血小板保留减少。这些发现表明，该柱是评估噻氯匹定对动脉粥样硬化疾病影响的良好工具。

Platelets have a crucial role in the maintenance of normal haemostasis, and perturbations of this system can lead to pathological thrombus formation and vascular occlusion, resulting in vascular accidents such as stroke, unstable angina and myocardial infarction. To prevent this pathological thrombus formation anti-platelet drugs have been developed, and are now widely used for prevention and therapy of vascular disorders. Among many methods for the evaluation of platelet functions and the evaluation of anti-platelet drugs on platelet functions, platelet adhesion and aggregation tests have been most frequently employed. They have met with limited approval in clinical settings, since they have several shortcomings including relative insensitivity to detect weak platelet activation, lack of reproducibility or clinical relevance, or tedious procedures for measurements. To overcome those disadvantages, we develop a new column which contains small-sized collagen beads.Small-sized collagen bead column comprise spherical copolymer-plastic beads coated with porcine type I collagen, packed into polyvinyl tubing. The blood was passed through the small-sized collagen beads column. The entire blood before and after passage through columns was collected and was counted platelets number. Platelet retention rates (%) were calculated as 100 x [(platelet count before the passage)-(platelet count after the passage)]/(platelet count before the passage).ADP scavengers and ADP receptor blockers but not aspirin appears to support platelet activation in this system. Blood samples drawn from healthy volunteers taking ticlopidine orally showed reduced platelet retention in the 'ADP columns'. These findings suggest that the column is a good tool for the assessment of ticlopidine effects on atherosclerotic diseases.