The study on the mechanism of production of autoantibodies in autoimmune hemolytic anemia.

Rh多肽，尤其是RhCcEe，是自身免疫性溶血性贫血(AIHA)常见的自身抗原。Rh血型抗原和AIHA自身抗原是构象抗原，由六个Rh多肽胞外环构成。通过筛选噬菌体展示的随机肽库，对自身抗原表位(模拟表位)的复制品进行鉴定。1.鉴定Rh模拟表位，反应抗D抗体和/或抗E抗体。2.鉴定自身抗原模拟表位，反应AIHA患者的自身抗体。3.8个自身抗体中有6个与Rh模拟表位发生反应，因此推测Rh模拟表位可能包含在自身抗原模拟表位中。4.这些模拟表位将为AIHA中自身抗体的鉴定和分类、Rh多肽上自身抗原表位的定位以及验证外来抗原与Rh多肽之间的分子拟合性成为AIHA自身抗体的病因。

Rh polypeptides, especially RhCcEe, are the common autoantigens in autoimmune hemolytic anemia (AIHA). Rh blood group antigens and the autoantigens in AIHA are conformational antigens and constructed by six extracellular loops of Rh polypeptides. Identification of replica of autoantigen epitopes (mimotope) was performed by means of screening phage displayed random peptide library.1.Identification of Rh mimotopes, reacting monoclonal anti-D antibody and/or anti-E antibody.2.Identification of autoantigen mimotopes, reacting autoantibody from AIHA patients.3.Six out of eight of the autoantibodies reacted the Rh mimotopes, therefore it is assumed that the Rh mimotopes might be included into autoantigen mimotopes.4.These mimotopes will make it possible to identify and categorize autoantibodies in AIHA, to map epitopes of autoantigens on Rh peptides and to verify molecular mimicry between foreign antigens and Rh polypeptides as the etiology of autoantibodies in AIHA.