Proliferation of human lymphoid malignancy by the Interaction of follicular dendritic cells

生发中心B细胞在滤泡树突状细胞（follicular dendritic cells， FDC）的帮助下分化，滤泡性淋巴瘤（follicular lymphoma， FL）也含有大量的FDC。为了明确FL与FDC之间的相互作用，我们将FDC样细胞系HK与FL共培养，HK不能充分支持FL细胞的生长，但在IL-2、IL-4、IL-7和IL-15的混合存在下，HK只支持FL中存在的CD4(+) T细胞的生长，一个月后细胞数量急剧增加了1000多倍。这些数据表明，在IL-2、IL-4、IL-7和IL-15存在的情况下，HK细胞支持CD4(+) T细胞的生长。然后，为了确认HK细胞是否刺激CD4(+) t淋巴瘤的生长，我们将3例成人t细胞白血病/淋巴瘤（Adult T-cell leukemia/lymphoma， ATLL）的淋巴结与HK和il共培养2,4,7,15。在所有三个病例中，CD4(+) t细胞增殖超过3个月。其中一个细胞是通过t细胞受体γ链重排模式从原始淋巴瘤细胞中获得的。这种ATLL衍生的细胞系依赖于HK，因为没有HK，它在几周后停止增殖并最终死亡。这些数据提示，在某些成人t细胞白血病/淋巴瘤中，FDC在与t细胞相互作用的过程中为其生长提供了有利的环境。为了证实其他t细胞淋巴瘤是否受到HK的刺激，我们将部分t细胞淋巴瘤细胞与HK细胞共培养。其中，三分之二的血管免疫母细胞t细胞淋巴瘤在IL-2、IL-4、IL-7和IL-15存在的情况下也在HK细胞上增殖。原淋巴瘤细胞的克隆带在生长细胞中保存。这些数据表明，在淋巴结中，fdc为几种恶性淋巴瘤的增殖提供了有利的环境。

Germinal center B cells are differentiated with the help of follicular dendritic cells(FDC), and follicular lymphoma(FL) also contain substantial FDC. To clarify the interaction between FL and FDC, FDC like cell line HK was co-cultured with FL. HK did not support the growth of FL cells sufficiently, however, in the presence of the mixture of IL-2, IL-4, IL-7, and IL-15,HK supported exclusively the growth of CD4(+) T cells which existed in FL. After a month, cell number highly increased more than 1000 folds. These data indicated that HK cells supported the growth of CD4(+) T cells in the presence of IL-2, IL-4, IL-7 and IL-15. Then, to confirm whether HK cells stimulate the growth of CD4(+) T-lymphoma or not, lymph node of three Adult T-cell leukemia/lymphoma(ATLL) were co-cultured with HK and IL-2,4,7,15. In all three cases, CD4(+) T-cells proliferate vigorously more than 3 months. One of three cells was derived from original lymphoma cells by using T-cell receptor gamma chain rearrangement pattern. This ATLL derived cell line is dependent on HK, because without HK, it stopped to proliferate a few weeks later and finally died. These data suggested that some of Adult T-cell leukemia/lymphoma, FDC provide the favorable environment for its growth in the course of the interaction of FDC and T-cells. In order to confirm whether other T-cell lymphomas are stimulated by HK or not, some T-cell lymphoma cells were co-cultured with HK cells. Among them, two of three angioimmunoblastic T-cell lymphoma also proliferated on the HK cells in the presence of IL-2, IL-4, IL-7 and IL-15. The clonal band of the original lymphoma cells was conserved in the growing cells. These data indicated in the lymph node, FDCs afford favorable environment to proliferate several types of malignant lymphoma.