Interaction between neuropeptides and S100 proteins in the folliculo-sebaceous unit

我们检测了S100基因的排列，一个S100基因在毛发周期中的表现漂移，神经肽P物质和前体ppt-A的表达，以及中性内肽酶在皮肤毛发-皮脂单位的定位。1.S100基因的排列：我们对含有S100A3基因的BAC进行了Southern杂交分析，以进行S100A3基因的基因组定位。通过与S100A3、S100A4和S100A6探针杂交，我们了解到在同一个BAC克隆上有三个基因排列在这三个基因中。在此基础上，我们获得了实验室小鼠三级染色体S100基因的限制性内切酶图谱。2.S100基因在毛发周期中的漂移：通过S100A反义RNA探针对毛发周期中表达水平的Northern杂交分析，S100A3的表现限制了它在生长期和人的…中的表达S100A4、A6在退化期/静止期/生长期(毛发周期的第一个转折点)表现更明显。3.结合S100A2、A4、A6蛋白在毛发粘液瘤中的表达，对22例毛发粘液瘤中S100A2、A4、A6蛋白的免疫组织化学表现进行了综述，并与正常毛囊中的表现进行了比较。毛囊间充质瘤的构成细胞部分显示不同的S100A蛋白表达，可见仍具有促进毛囊绒毛生长的分化潜能。4.由于ICR实验小鼠皮肤中有P物质和中性内肽酶的免疫染色，对于P物质，毛囊/皮脂腺系统中的P物质与周围结缔组织的神经纤维呈阳性而不显露。而中性内肽酶则在毛囊和皮脂腺边缘细胞的外根鞘中呈阳性反应。S100A3与角质层细胞呈强阳性反应，对毛皮细胞呈弱阳性反应。此外，S100A6主要在内根鞘中发现阳性结果。经P物质/中性内切酶染色和S100A3、A4、A6双重染色，发现两者在毛发周期各时间点的定位均一致。较少

We examined arrangement of S100 gene, manifestation drifting of a S100 gene in hair cycle, expression of neuropeptide substance P and the precursor ppt-A, and localization of protein levels of neutral endopeptidase in the folhculo-sebaceous unit in skin. In addition, we examined manifestation of S100A protein in a pilomatrixoma.1.An arrangement of a S100 gene : We did a southern blot analysis of a BAC done including a S100A3 gene to do mapping in genome of a S100A3 gene. As a result of having hybridized S100A3, S100A4 and S100A6 probe, we understood that three genes arranged it in these on the same BAC clone. Based on these results, we obtained a restriction endonudease map of S100 gene duster of a laboratory mouse tertiary chromosome.2.Manifestation drifting of a S100 gene in hair cycle : As a result of having done Northern blot analysis about mRNA level of expression in hair cycle using antisense RNA probe for S100A mRNA, manifestation of S100A3 confined it for growth period, and man … More ifestation was found in S100A4, A6 for involution/resting phase/growth period (the first hair cycle turning point).3.We reviewed it because of manifestation of S100A2, A4, A6 protein in pilomatrixoma 22 examples immunohistologically and, about a differentiation tendency of this skin tumor, compared it with manifestation in normal folliculus pili. A constitution cell of a pilomatrixoma showed manifestation of different S100A protein partially, and it became clear to still have the differentiation potency which contributed to folliculus pili build-up.4.As a result of having immunostained substance P in ICR laboratory mouse skin and neutral endopeptidase, as for substance P, the manifestation in folliculus pili/glandulae sebaceae system showed positive to a nerve fiber of circumference connective tissue without revealing it. On the other hand, as for neutral endopeptidase, positive findings were found in outer root sheath of some folliculus pili and glandulae sebaceae marginal cell. S100A3 was strongly stained with a cuticle cell and revealed weak manifestation to a hair cortex cell. Furthermore, S100A6 mainly showed positive findings in inner root sheath. By substance P/neutral endopeptidase and double stain of S100A3, A4, A6 each, the identity was found in localization of both in neither time of hair cycle. Less