Biological modulation of steroid for bile output

1.腹腔注射1.6 mg地塞米松可显著增加大鼠肝脏基础胆汁排出量，地塞米松组为2.495 ±0.299 μL/min/g肝脏对照组为2.152 ±0.083 μL/min/g肝脏对照组P = 0.001622。硫化氢是在肝脏中通过半胱氨酸代谢产生的。在大鼠肝脏中，组织硫化氢含量约为80 μmol/g组织，首次采用气相色谱法测定; 3.腹腔注射CSE抑制剂- (1)In PPG组基础胆汁排出量较对照组明显增加。（2）在PPG处理的大鼠肝脏中补充NaHS完全抑制PPG对大鼠肝脏胆汁分泌的影响。（3）PPG处理组基础胆汁排出量的增加是由于胆汁碳酸氢盐排泄量增加所致。硫化氢是肝脏基础胆汁生成的内源性气体调节剂。它调节胆汁碳酸氢盐排泄。

1.Peritoneal administration of steroid, 1.6 mg-dexamethazone, significantly increased basal biliary output in perfused rat liver.2.495±0.299 μL/min/g liver dexamethazone group2.152±0.083 μL/min/g liver control groupP=0.001622.Hydrogen sulfide is generated through cysteine metabolism with the catalytic enzyme such as cytathionine γ-lyase (CSE) in the liver. In the rat liver, the content of tissue hydrogen sulfide was around 80 μmol/g tissue, which was firstly measured by our gas-choromatography technique.3.Propagylglycine (PPG) administrated intraperitoneally, which was a potent inhibitor of CSE, significantly decreased tissue hydrogen sulfide level.4.The bile output and its constituents were evaluated in both in vivo and ex vivo perfusion system.(1)In the PPG-treated liver, basal biliary output significantly increased compared with that of control liver.(2)Supplementation of NaHS in the PPG-treated liver completely inhibited the PPG-effect on bile output in the prefused rat liver.(3)The elevation of basal bile output in the PPG-treated liver resulted from the increased excretion of biliary bicarbonate. And supplementation of NaHS also inhibited this PPG-effect.Hydrogen sulfide works as an endogenous gaseous modulator of the basal bile formation in the liver. And it modulates biliary bicarbonate excretion.