Study of expression mechanism of laminin-5 γ2 chain in colon cancer cell lines

MMP-7和层粘连蛋白-5 γ2链在癌细胞系中的表达（1）在L-10和HT-29细胞中，MMP-7和层粘连蛋白-5 γ2链均在癌细胞的细胞质中表达，而在SW 620、Colo 320 D、LS 174 T细胞中，这些蛋白不表达。队列迁移模型（2）如Nabeshima等人所建议的，队列迁移模型实际上在L-10细胞中观察到，然而，在所检查的其它细胞系中未观察到。L-10细胞在组织培养载玻片上形成相互连接、堆积的细胞岛，部分癌细胞从细胞岛向外迁移到岛间空间，形成一个细胞厚的连贯细胞片。HGF/SF刺激实验（3）重组肝细胞生长因子/分散因子（HGF/SF）刺激L-10细胞后，L-10细胞迁移数明显增加，抑制实验（4）L-10细胞在抗hMMP-7（+）培养液中培养时，MMP-7和LN-5 γ2链均表达于细胞浆中，MMP-7和LN-5 γ2链表达于细胞浆中。以与在普通培养基中培养时相同的方式观察到群体迁移。MMP-7和LN-5 γ2链的表达模式也相似。

MMP-7 and laminin-5 γ2 chain expression in cancer cell lines(1) In L-10 and HT-29 cells, both MMP-7 and laminin-5 γ2 chain were expressed in the cytoplasm of cancer cells, whereas in SW620, Colo320D, LS174T cells these proteins were not expressed.Cohort migration model(2) As Nabeshima et al.suggested, cohort migration model was actually observed in L-10 cells, however, it was not observed in the other cell lines examined. L-10 cells formed interlinked, piled-up cell islands on the tissue culture glass slides, and some cancer cells migrated outward from the cell islands into inter-island spaces as coherent cell sheets with one-cell thick. Both MMP-7 and laminin-5 γ2 chain were expressed in the cytoplasm of migrated cancer cellsHGF/SF stimulation test(3) When stimulated with recombinant hepatocyte growth factor/scattered factor (HGF/SF), the number of migrated L-10 cells were significantly increased as compared with that without stimulation.Suppression test(4) When L-10 cells were cultured in the anti-h MMP-7 (+) medium, cohort migration was observed as in the same fashion as when cultured in the ordinary medium. The expression patterns of MMP-7 and laminin-5 γ2 chain were also similar.