Gale therapy for osteoarthritis

1.为了确定IL-4介导的腺病毒载体对小鼠骨关节炎模型的治疗作用，我们将AdCIL4静脉注射给16周龄雄性SRT/ort小鼠。我们观察到，注射AdCIL4的小鼠的膝关节与对照组相比，骨关节炎改变的影响明显受到抑制。虽然存在AdCIL4诱导炎症的问题，但似乎载体局部给药是治疗骨软骨病变的一种方法。目的探讨IL-4对体外过度机械应力下软骨细胞基质合成的影响。机械应激16小时显著降低AGG和CII mRNA水平（p<0.01），但1或10 ng/ml剂量的大鼠IL-4恢复了这些水平（p<0.05）。这些发现表明，IL-4可能防止关节软骨由机械应力引起的损伤，因此可能是治疗关节炎疾病的有效药物。

1.To determine the therapeutic effect of IL-4 mediated adenovirus vector on the murine osteoarthritis model, AdCIL4 was administrated to the male 16-week-old SRT/ort mice intravenously. It was observed that knee joints form the mice to which injected with AdCIL4 showed significantly inhibited to affect with osteoarthritic changes compared with those of control mice. Although there was the problem to induce the inflammation by AdCIL4, it seemed that local administration with vectors could be a therapeutic method for osteochondral lesion.2.To examine the effect of IL-4 on matrix synthesis in chondrocytes under excessive mechanical stress in vitro. Mechanical stress for 16 hours significantly decreased levels of mRNA for both AGG and CII (p<0.01), but rat IL-4 at doses of 1 or 10 ng/ml recovered these levels (p<0.05).These findings suggested that IL-4 might prevent articular cartilage from damage caused by mechanical stress and therefore could be a useful therapeutic agent for treatment of arthritic disorders.