Participation of the sympathetic nervous system in formation of the bed sore : Neuroanatomical research using the animal model.

2003年，我研究了大鼠汗腺支配TH、VIP、CGRP、VAChT和SP等神经递质的神经纤维的分布规律，发现TH、VIP、CGRP和VAChT等神经纤维密集分布于汗腺。另一方面，在汗腺中未观察到包括神经纤维在内的SP，而主要存在于汗腺周围的血管中。在2004年，我试图在2003年结果的基础上比较褥疮区域和对照区域的神经纤维分布模式。然而，我没能在老鼠的爪垫上弄痛床。我第一次尝试用弹簧夹来做褥疮。夹紧后不久，我无法观察到爪垫的任何变化。夹闭后6小时出现水肿，我观察到夹闭点周围区域血流障碍引起的皮肤颜色变化。在组织学检查中，在湿化后6小时使用HE染色，我可以观察到皮下区域的水肿变化和许多炎性细胞。免疫组化检查，使用抗PGP 9 - 5抗体，这是已知的染色几乎所有的神经纤维，显示没有阳性染色。这意味着支配汗腺的神经纤维被夹伤，因此，我无法在项目期间成功地制作褥疮。我将尝试使用另一种动物模型，suncus，在身体的所有区域都有汗腺，以明确褥疮区域汗腺神经支配模式的变化。

In 2003, I investigated the distribution pattern of rats sweat gland innervating nerve fibers including several neurotransmitters, that is, TH, VIP, CGRP, VAChT and SP Nerve fibers including TH, VIP CGRP and VAChT were distributed densely in the sweat gland. On the other hand, SP including nerve fibers were not observed in the sweat gland, but were mainly existed at blood vessels surrounding the sweat gland. Somas extending these fibers were existed in the stellate ganglion and spinal ganglion.In 2004, I tried to compare the distribution pattern of nerve fibers in the region of the bed sore with the control region based on results of 2003. However, I failed to make the bed sore at paw pads of the rat. I firstly tried to make bed sore using clamp with spring. I could not observe any change in paw pads soon after clamping. Edemas were showed at 6 hours after clamping and I observed change of the skin color because of the disturbance of blood flow in the peripheral region of clamping point. In histological examination using HE staining at 6 hours after damping, I could observe edematous changes and many inflammatory cells in the subcutaneous region. Immunohistochemical examinations using anti PGP 9 5 antibody, which are known to stain almost all nerve fibers, showed no positive staining. This meant that nerve fibers innervating sweat glands were damaged by the clamping Thus, I could not make the bed sore successfully between the term of project.I will try to make bed sore using another animal model, suncus, which have sweat glands in all region of the body, and make clear the change of innervation pattern of sweat gland in bed sore regions.