Role of tachikinin which release from C-fiber endings on cough sensitivity in chronic coughs.

1.用局限曲霉菌株A-17的蛋白组分被动致敏引起的咳嗽过敏我们检测了用局限曲霉菌株A-17的蛋白组分被动致敏的豚鼠对吸入柠檬酸的咳嗽反射的敏感性。与非致敏组相比，致敏组中5%柠檬酸气雾剂引起的咳嗽次数显著增加。当动物在吸入柠檬酸前60 min用选择性组胺H1受体拮抗剂非索非那定预处理时，致敏豚鼠中柠檬酸诱导的咳嗽次数呈剂量依赖性并显著降低至非致敏豚鼠的水平。与非致敏组相比，致敏组的支气管对吸入乙酰甲胆碱或组胺的反应性没有显著升高。这些结果表明，用A.限制性增强 ...更多信息 2.维生素D转运体抑制剂VDM 11对辣椒素诱导的小鼠咳嗽的影响维生素D转运体是否参与了辣椒素诱导的小鼠咳嗽的调节尚不清楚。为了验证这一假设，我们研究了VDM 11（一种大麻素膜转运蛋白抑制剂）对辣椒素和大麻素诱导的咳嗽的影响。检查了VDM 11（一种大麻素膜转运蛋白抑制剂）对辣椒素和大麻素诱导的小鼠咳嗽的作用。3-10 mg/kg剂量的VDM 11皮下给药产生剂量依赖性止咳作用。这种镇咳作用被预先腹腔注射（3 mg/kg）或吸入（1 mg/ml）大麻素受体（CB 1）拮抗剂SR 141716 A所拮抗。当辣椒平（0.3 mM），一种选择性TRPV 1受体拮抗剂，通过气雾剂给药4分钟，然后吸入3 mg/ml的花生四烯酸，咳嗽次数显着减少到单独使用10%DMSO观察到的水平。这些结果表明，花生四烯酸，内源性大麻素配体，可能会调节咳嗽敏感性和花生四烯酸转运蛋白在这种调制中发挥重要作用。少

1.Cough hypersensitivity caused by passive sensitization with protein fraction of Aspergillus restrictus strain A-17We examined the sensitivity of the cough reflex to inhaled citric acid in guinea pigs that had been passively sensitized with the protein fraction of Aspergillus restrictus strain A-17. The number of coughs elicited by an aerosol of 5 % citric acid was significantly increased in the sensitized group compared to the non-sensitized group. The number of citric acid-induced coughs in sensitized guinea pigs was dose-dependently and significantly reduced to the level in non-sensitized guinea pigs when animals were pretreated with fexofenadine, a selective histamine H1 receptor antagonist, 60 min before citric acid inhalation. The bronchial responsiveness to inhaled methacholine or histamine in the sensitized group was not significantly heightened compared to the non-sensitized group. These results suggest that passive sensitization with the protein fraction of A. restrictus enh … More ances the excitability of cough receptors to tussive stimuli, and the physiologic features of this animal model are consistent with those of atopic cough.2.Effect of VDM11, an anandamide transporter inhibitor, on capsaicin-induced cough in miceIt is unknown whether the anandamide membrane transporter plays a role in this modulation. To test this hypothesis, we investigated the effects of VDM11, an anandamide membrane transporter inhibitor, on capsaicin- and anandamide-induced cough. The effect of VDM11, an anandamide membrane transporter inhibitor, on capsaicin- and anandamide-induced cough in mice was examined. VDM11, at doses of 3-10 mg/kg subcutaneously, produced a dose-dependent antitussive effect. This antitussive effect was antagonized by pretreatment with either intraperitoneal administration (3 mg/kg) or inhalation (1mg/ml) of SR141716A, a cannabinoid receptor (CB1) antagonist. When capsazepine (0.3 mM), a selective TRPV1 receptor antagonist, was given via aerosol for 4 min before inhalation of 3 mg/ml of anandamide, the number of coughs was significantly decreased to the levels observed with 10% DMSO alone. These results suggest that anandamide, an endogenous cannabinoid ligand, may modulate cough sensitivity and that anandamide transporters play an important role in this modulation. Less