Functional analysis of enhanced adaptor molecule Shc in the nuclei of the hepatocellular carcinoma

Shc蛋白与细胞增殖和癌变有关。然而，Shc在肝细胞癌中的参与仍然未知。令人惊讶的是，p52 Shc仅定位于肝细胞和癌细胞的细胞质中，而p46 Shc在正常肝细胞和癌细胞的细胞核中表达。此外，p46 Shc和p52 Shc在肝癌中的表达高于同一患者的邻近正常肝细胞。提示p46 Shc和p52 Shc的增强可能与肝癌的发生有关。此外，与p52 Shc不同，p46 Shc在具有高增殖活性的肝细胞癌细胞核中表达。因此，p46 Shc在肝癌细胞核中的表达可能在肝癌的发生发展中起重要作用。在结肠癌和胃癌中也观察到这些事件。

Shc protein is known to be related to cell proliferation and carcinogenesis. However, the involvement of Shc in hepatocellular carcinoma remains unknown. Surprisingly, p52 Shc was localized only in the cytoplasm of hepatocytes and cancer cells, while p46 Shc was expressed in the nuclei of normal hepatocytes and cancer cells. In addition, the expression of p46 Shc and that of p52 Shc were higher in hepatocellular carcinoma than in adjacent normal hepatocytes from the same patient. These results suggest that the enhancement of p46 Shc and p52 Shc may be related to the occurrence of hepatocellular carcinoma. Furthermore, unlike p52 Shc, p46 Shc was expressed in the nuclei of hepatocellular carcinoma cells with high proliferative activity. Therefore, p46 Shc expressed in the nuclei may play an important role in the progression of hepatocellular carcinoma. These events were also observed in colon cancer and gastric cancer.