Establish of the animal model of erythema multiforme induced by herpes simplex infection.

我们构建了含有角蛋白14启动子、人单纯疱疹病毒糖蛋白B（HSV-g B）和内含子/ poly A信号的表达载体。将该构建体转染Hela细胞，用HSV-gB多肽免疫家兔，制备多克隆抗体，检测HSV-gB在Hela细胞中的表达，并利用该构建体建立皮肤特异性表达HSV-gB蛋白的转基因小鼠。将构建体显微注射到受精卵中并转移到假孕小鼠的输卵管中。获得119只小鼠。目前我们已获得至少5只经PCR检测携带HSV-gB基因的转基因小鼠，将在皮肤中检测HSV-gB基因的表达，并将这些转基因小鼠感染单纯疱疹病毒I型。监测临床症状和生存率。将通过细胞毒性T细胞应答（γ <51>Cr掺入试验）、CD 4 T细胞应答（γ 3 H掺入试验）测量免疫应答。

We constructed the expressoin vector which contains keratin 14 promotor, human herpes simplex virus glycoprotein B (HSV-gB) and intron/ poly A signal. This construct were transfected into Hela cells and HSV-gB expression in those cells was confirmed by polyclonal antibody which was established from rabbit immunized with HSV-gB peptides.Using this construct, we are trying to generate transgenic mice which selectively express HSV-gB protein in skin. The construct were microinjected into fertilized eggs and transferred into oviduct of psuedopregnant mouse. 119 mice were obtained. We now obtaine at least 5 founders carrying the introducing HSV-gB gene detected by PCR.The expression of HSV-gB will be checked in skin and these transgenic mice will be infected with herpes simplex virus type I. We monitor the clinical sympton and survival rate. The immunological response will be measured by cytotoxic T cell response (^<51>Cr reraese assay), CD4 T cell response (^3H incorporation assay).