Establishment of database of IFN-α production test and risk management of hepatocellular carcinoma in patients with hepatitis C virus.

在此之前，我们在World J Gastroenterology杂志上发表了一篇题为“丙型肝炎病毒患者IFN-α生产能力受损与肝细胞癌发展的风险”的论文，这是本项目的基础。该项目需要路易斯巴斯德医学研究中心建立一个跨越17年的IFN-α产生和血液测试数据库。IFN-α产生的数据是通过对2315名研究人群的医疗记录进行回顾性分析获得的，其中选择了112名健康对照者和20名hcv感染患者。60%的hcv感染患者的IFN-α产生受损或下降，而健康对照组的这一比例为17%。发生肝细胞癌患者的平均IFN-α水平低于未发生癌症的hcv感染患者。这些结果发表在IFN和细胞因子研究杂志上，题为“干扰素-a产生受损和癌症发展的风险”。在此基础上，建立了仙台病毒刺激法检测IFN-α产生细胞的方法，发现IFN-α产生细胞主要为浆细胞样树突状细胞。这种方法将有助于理解IFN-α产生受损的分析。

Previously, we published a manuscript entitled "Impairment of IFN-α production capacity in patients with hepatitis C virus and the risk of the development of hepatocellular carcinoma" in World J Gastroenterology, which forms the basis of this project. The project entailed the establishment of a database of IFN-α production and blood tests spanning 17 years by the Louis Pasteur Center for Medical Research. Data for IFN-α production was obtained retrospectively over a 17-year period by examining medical records in a study population of 2,315 individuals, of which 112 healthy controls and 20 HCV-infected patients were selected. Sixty percent of the HCV-infected patients had impaired or declining IFN-α production in comparison to 17% in the healthy control group. Mean IFN-α levels were lower in patients who developed hepatocellular carcinoma than in the HCV-infected patients who remained cancer-free. These results have been published in J IFN ＆ Cytokine Research as "Impaired interferon-a production and the risk of cancer development." Further, we established the methodto detect IFN-α producing cells by Sendai virus stimulation using FACS, and the main IFN-α producing cells were plasmacytoid dendritic cells. This method will be useful to understand the analysis of impaired IFN-α production.