Dissecting the molecular interplay between tumor cells and stroma in primary liver cancer (B08)

项目B08(Longerich/Pellegrino；解剖原发性肝癌中肿瘤细胞和间质之间的分子相互作用)已于2018年底进入SFB/TR209，分析肝癌细胞可塑性的潜在机制，这与肿瘤分化和治疗耐药有关。该项目利用不同肝癌变异体的外显子组/转录组测序，通过作用于肿瘤细胞和/或肿瘤相关间质，确定了影响肝癌细胞分化/可塑性的候选基因。在新的资助期内，将在马赛克小鼠模型中对功能获得表型驱动因素候选进行评估，并在体外和体内进行功能表征，目的是确定肿瘤细胞可塑性和肿瘤相关间质之间的相互依赖模式和潜在的干预方法。

Project B08 (Longerich/Pellegrino; Dissecting the molecular interplay between tumor cells and stroma in primary liver cancer) has entered the SFB/TR 209 in late 2018 and analyses mechanisms underlying plasticity of liver cancer cells, which bear on tumor differentiation and therapy resistance. The project has identified candidate genes affecting liver cancer cell differentiation/plasticity by acting on tumor cells and/or tumor-associated stroma using exome/transcriptome sequencing of different liver cancer variants. In the new funding period the gain-of-function phenotypic driver candidates will be evaluated in mosaic mouse models and functionally characterized in vitro and in vivo with the aim to identify modes of interdependency between tumor cell plasticity and tumor-associated stroma and potential ways of intervention.