Global analysis of symbiotic tissue specific gene expressions of nitrogen and carbon metabolisms in legume nodules

1)对大豆根瘤线粒体和根瘤类细菌的蛋白质组学分析进行了综述。结节线粒体的2D-EP蛋白质量指纹图谱和LCMSMS分析显示，465个斑点（结节线粒体）和383个斑点（根线粒体）是组织特异性的。利用Edman降解法、蛋白质量指纹图谱和LCMSMS分析对这些蛋白斑点进行鉴定。虽然数据库中有很多功能点没有登记，但数据显示鉴定出7个上调蛋白和8个下调蛋白。在结节线粒体中还发现了6种抑制蛋白。讨论了这些蛋白在结核代谢中的作用。类似的方法也适用于结核类细菌。在这个类细菌蛋白质组实验中，我们鉴定出62个在结核类细菌中与培养细菌相比上调的蛋白质。2)为了准备基因组测序数据，我们加入了Kazusa DNA研究所项目组和Kouchi博士的实验室。在这个实验中，我们从克隆的5'位点对9000多个cDNA克隆进行了测序，这些数据将通过Kazusa DNA研究所向公众提供。

1)Proteome analysis of nodule mitochondria and nodule bacteroids of soybean plants was surveyed, In. the case of nodule mitochondria 2D-EP following Protein Mass Finger print and LCMSMS analysis revealed that 465 spots (nodule mitochondria) and 383 spots (root mitochondria) were tissue specific. Using Edman degradation methods, Protein Mass Finger print and LCMSMS analysis identification of these protein spots were performed. Although there are many spots which the function were not registered in the database, the data showed 7 up-regulating and 8 down-regulating proteins were identified. Six suppressed proteins in nodule mitochondria were identified too. The role in nodule metabolism of these proteins were discussed. Similar approach was employed in the case of nodule bacteroids too. In this bacteroid proteome experiment, we identified 62 proteins which were up-regulated in nodule bacteroids comparing to cultured bacteria.2)For preparing genome sequencing data we joined to the project team of Kazusa DNA Research Institute and Dr. Kouchi' laboratory. In this experiment we sequenced more than 9,000 cDNA clones from 5'site of the clone and the data is going to supplied to the public through Kazusa DNA Research Institute.