Studies on the development of fucoidan prepared from commercially cultured Cladosiphon okamuranus TOKIDA(Okinawamozuku)

我之前曾从商业养殖的冈村枝管鱼(Okinawamozuku)中分离出一种岩藻糖胶，其产量估计为2.0-2.3%(湿海藻)。褐藻糖胶具有降胆固醇、抗凝血、抗肿瘤等生理生物活性。本文报道了从日本冲绳岛南部(池嫩村)海上渔网上商业化养殖的冲绳目鱼制备的岩藻糖胶的流变性和抗凝血活性。岩藻糖胶由L-岩藻糖：D-木糖：D-葡萄糖醛酸：醋酸：硫酸=4.0：0.1：1.0：2.0：2.0组成。用同轴柱和旋转外柱组成的流变仪测定了岩藻糖胶的非牛顿行为和动态粘弹性。在25℃时，岩藻糖胶的流动曲线在1.5%以下表现为剪切变稀行为，而在2.0%时表现为塑性行为。在不同浓度的岩藻糖胶溶液中，动态黏度略高于储能模数。在2.0%岩藻糖胶溶液中加入0.1%的氯化钠和0.1%的氯化钙，动态粘弹性增大。岩藻糖胶的动态粘弹性在较宽的pH范围内是稳定的。岩藻糖胶在水溶液中具有独特的流变学特性，有望应用于食品、化妆品等行业。凝血酶和活化的凝血活酶活性高于肝素。经硫酸和甲酸部分水解的岩藻糖胶比天然岩藻糖胶具有更高的活性。

I have previously isolated a fucoidan from commercially cultured Cladosiphon okamuranus TOKIDA(Okinawamozuku), the yield of which was estimated to be 2.0-2.3%(wet seaweed). Fucoidan has physiological and biological activities of an cholesterol lowering, anti-coagulant and anti-tumor agent. I report herein rheological properties and blood anti-coagulant activity of the fucoidan prepared from commercially cultured Okinawamozuku on nets at sea off the southern part of Okinawa Island(Chinen Village),Japan.The fucoidan consisted of L-fucose:D-xylose:D-glucuronic acid:acetic acid:sulfuric acid=4.0:0.1:1.0:2.0:2.0. The molecular weight of the fucoidan was estimated to be 500,000.The non-Newtonian behavior and dynamic viscoelasticity of the fucoidan were measured with a rheogoniometer equipped with a coaxial cylinder and rotating outer cylinder. The flow curves of the fucoidan, at 25℃, showed shear-thinning behavior below 1.5%, but plastic behavior at 2.0%. A little higher dynamic viscosity was observed than that of storage modulus in fucoidan solutions at various concentrations. The dynamic viscoelasticity increased with addition of NaCl(0.1%) and CaCl_2(0.1%) to 2.0% fucoidan solution. The dynamic viscoelasticity of the fucoidan was stable over a wide pH range. The fucoidan had unique Theological characteristics in aqueous solution, therefore it migh be able to use in foods, cosmetics and some other industries.Blood anti-coagulant activity tests were conducted on th fucoidan with human plasma in comparison with heparin. The thrombin and activated thromboplastin activity was higher than that of heparin. Partially hydrolyzed fucoidans with sulfuric acid and formic acid had higher activity that that of native fucoidan.