OXIDATIVE STRESS AND EXERCISE : ESTABLISHMENT OF EXERCISE THERAPY FOR HEALTH PROMOTION AND PREVENTION OF DIABETES

1.氧化应激蓄积的新标记物我们建立了红细胞膜上的氧化胆固醇3，5，7-胆三烯(3，5，7-C)的GC/MS检测体系和用ELISA法提取的羧甲基缬氨酸血红蛋白(CMV-Hb)的检测体系。我们发现这些新的标记物反映了几个月来氧化应激的积累，并且在糖尿病患者中增加。2.氧化应激相关基因多态性与糖尿病和动脉粥样硬化的关系我们评估了先前报道的10个与氧化应激相关的SNP，发现NAD(P)H氧化酶p22Phox C242T和ACE I/D基因的组合与动脉粥样硬化相关。我们应该进一步研究发现与糖尿病或动脉粥样硬化相关的氧化应激相关的新的SNP。3.体脂分布的精细评估我们用1H-MRS检测了肌内脂肪(IMCL)和肝内脂肪(IHL)，并用腹部MRI检测了内脏脂肪体积(VF)。4.生活方式改变对非糖尿病肥胖者的干预我们评估了非糖尿病肥胖者不增加3，5，7-C和CNV-Hb(150-170千卡+日常生活)和限制能量(30千卡/IBW公斤)运动6-12个月的效果。改善IMCL、HL、VF和血脂谱，改善肝脏和肌肉的胰岛素敏感性。我们应该制定个性化的最佳运动和饮食方案，并对其效果进行评估，以供进一步研究。

1.New marker of oxidative stress accumulationWe established the assay systems of 3,5,7-cholestatriene (3,5,7-C), a kind of oxycholesterol in RBC membrane, by GC/MS and carboxymethyl-valine hemoglobin (CMV-Hb) extracted from RBC by ELISA. We revealed that these new markers reflected accumulation of oxidative stress for several months and they increased in diabetic patients.2.Association of oxidative stress-related gene polymorphism with diabetes and atherosclerosisWe evaluated previously reported ten SNP related to oxidative stress and found that the combination of NAD(P)H oxidase p22phox C242T and ACE I/D genotypes was associated with atherosclerosis. We should further investigate to find new SNP related to oxidative stress associating with diabetes or atherosclerosis.3.Fine assessment of body fat distributionWe investigated intramyocellular lipid (IMCL) and intrahepatic lipid (IHL) by 1H-MRS and visceral fat volume (VF) by abdominal MRI. We found that IMCL, IHL, and VF could be reduced by short term life style modification before reduction of BW in non-diabetic obese subjects and type 2 diabetic patients.4.Intervention of life style modification on non-diabetic obese subjectsWe evaluated the effect of exercise not increasing 3,5,7-C and CNV-Hb (150-170 kcal+daily life) and energy restriction (30 kcal/IBW kg) on non-diabetic obese subjects for 6-12 months. IMCL, HL,VF, and serum lipid profile were ameliorated, and insulin sensitivity in liver and muscle were improved. We should make individualized program of optimal exercise and diet, and evaluate its effect for further study.