RESEARCH FOR PSEUDOCAPSULE FORMATION OF METASTATIC LIVER TUMORS

目的：通过对结直肠癌肝转移瘤的病理形态和DNA芯片的分析，探讨肝肿瘤周围假包膜形成的原因和发病机制。材料和方法：1.从结直肠癌切除的肝肿瘤冰冻标本中提取组织RNA和DNA芯片分析，用激光显微切割法从假包膜附近的癌组织和非癌肝实质中提取总RNA。分别选择肿瘤包膜较厚的5例和肿瘤无包膜的5例进行分析。采用GeneSpring和d-Chip软件进行统计学分析。结果：从GeneSpring软件分析可知，可能与伪囊形成相关的基因在癌组织中比在正常组织中更常见。D-Chip挑选了47个基因，这些基因显示了癌症组织和非癌症组织之间的不同表达水平。

PURPOSE : To clarify the cause and pathogenesis of pseudocapsule around the liver tumor, liver metastatic tumor from colorectal cancer were analyzed pathomorphologically and by DNA microarray. MATERIAL AND METHODS : 1.Harvesting of tissue RNA and DNA microarray analysis From the frozen specimen of resected liver tumor from colorectal cancer, total RNA was collected by Laser microdissection from cancer tissue near the pseudocapsule and noncancerous liver parenchyma. Five patients whose tumor had thick capsule and the other five whose tumor showed no capsule were selected for analysis, respectively. For analysis, GeneSpring and d-Chip software was used. RESULTS : From the analysis using GeneSpring software the genes possibly related to pseudocapsule formation were more frequently found in the cancer tissue than in the normal tissue. The d-Chip picked out 47 genes which showed the different level of expression between cancer and noncancerous tissues.