GENE AMPLIFICATION OF ANDROGEN RECEPTOR REGION IN HORMONALLY RESISTANT PROSTATE CANCER

用LNCaP、PC-3和DU145细胞株进行XQ12区基因扩增的研究。Xq12区的探针由AR和STS组成。与正常对照细胞系相比，LNCaP细胞株AR基因扩增。我们曾试图用无雄激素培养的方法从LNCaP中建立雄激素非依赖性亚克隆系，但以失败告终。低雄激素(R1881 1 ng/ml)培养LNCaP时，AR基因扩增倍数是正常雄激素培养LNCaP的1.2~1.8倍。上述资料提示AR基因扩增与雄激素非依赖性前列腺癌的发生有一定关系。

Study on gene amplification of Xq 12 region with LNCaP,PC-3 and DU145 cell lines.Southern blot was performed for DNA gained from these cell lines. Probes were composed of AR and STS in Xq12 region. Amplification of AR gene was observed in LNCaP compared with normal control cell lines. We had attempted to create androgen independent subclonal lines from LNCaP with cultivating under androgenfree medium, but in vain. When we cultivated LNCaP in low androgen medium(R1881 1ng/ml), AR gene showed 1.2〜1.8 times more amplification in comparison with LNCaP cultured under normal androgen concentration. Data described above suggested that amplification of AR gene had some relation to the development of androgen independent prostate cancer.