The induction of cytotoxic(killer) T lymphocytes against the tumor of neck by new culture method.

我们研究了体外混合淋巴细胞培养(MLC)诱导的细胞毒性T淋巴细胞(CTL)的特异性。我们采用独特的培养系统，将应答小鼠的骨髓(BM)细胞作为辅助细胞加入到含有应答淋巴结细胞和同种异体刺激脾细胞的培养中。本研究从小鼠脾细胞中分离出两种不同的树突状细胞(DC)，DEC-205+细胞去除(MDC)或MEC-1+细胞去除(LDC)。在allo-MLC中，MDC刺激诱导的CTL对刺激细胞的主要组织相容性抗原复合体(MHC)和X染色体连锁基因产物(XLGP)具有双重特异性。在刀豆蛋白A诱导的多克隆CTL中，以自体的、非同种异体的脾细胞作为刺激细胞。MDC刺激诱导的CTL具有双重特异性，如多克隆的allo-MHC和刺激物自身的XLGP。相反，当使用LDC刺激时，具有双重特异性的CTL被特异性地抑制。我们已经报道，用这些培养体系刺激来自DBA/2小鼠的P815肿瘤细胞诱导的CTL具有双重特异性，即H-2d(DBA/2小鼠的MHC)和P815肿瘤特异性抗原(TSA)。P815 TSA与XLGP相似，但不同，但具有与XLGP相同的抗原功能。本研究结果提示，某些(MDC类)肿瘤细胞可诱导具有MHC和XLGP样TSA双重特异性的CTL，而另一些(LDC类)肿瘤细胞可抑制或耐受具有这种双重特异性的CTL诱导。

We studied the specificity of cytotoxic T lymphocytes(CTLs) which were induced in mixed lymphocyte cultures(MLC) in vitro. We used the unique culture system that bone marrow(BM) cells from responder mice as accessory cells were added to the cultures, which contained both responder lymph node cells and allogeneic stimulator spleen cells. In this report, two different stimulatory dendritic cell(DC) populations were prepared from spleen cells, such as DEC-205+ cell depleted(Mac-1+ cell enriched;MDC) or Mac-1+ cell depleted(DEC-205+ cell enriched;LDC) spleen cells.In allo-MLC, the CTL induced by the stimulation of MDC had the dual specificity for both allo major histocompatibility antigen complex(MHC) and allo X-chromosome linked gene products(XLGP) of stimulator cells. On the other hand, the CTL induced by the stimulation of LDC had the specificity for allo MHC alone.In polyclonal CTL induction by concanavalin A, autologous, not allogeneic, spleen cells were used as stimulator cells. The CTL induced by MDC-stimuli had the dual specificity, such as polyclonal allo-MHC and stimulator-self-XLGP. On the contrary, when LDC-stimuli was used, the CTL having the dual specificity were specifically suppressed.We already reported that the CTL induced by the stimulation with P815 tumor cells(originated from DBA/2 mice), using these culture systems, had the dual specificity, that is, H-2d(MHC of DBA/2 mice) and P815 tumor specific antigens(TSA). P815 TSA behaved like the XLGP, but were different one.However, this TSA had the same antigenic function with XLGP. The conclusion in this report suggested the possibility that some(MDC type) tumor cells could induce the CTL having dual specificity, MHC and XLGP-like-TSA, the other hand, other(LDC type) tumor cells could suppress or tolerate the induction of CTL having this dual specificity.