Basic study for development of a novel periodontal tissue regeneration therapy utilizing immobilized cell growth factors

应用固定肝素的碱性成纤维细胞生长因子-2对牙周膜细胞进行苦参碱作用的研究。在胎牛血清存在下，肝素结合的成纤维细胞生长因子-2促进牙周膜细胞的增殖，抑制碱性磷酸酶活性和I型胶原的产生。将成纤维细胞生长因子-2和胎牛血清直接固定在组织培养板上也能上调牙周膜细胞DNA合成，下调碱性磷酸酶活性。这些结果表明，固定化生长因子如成纤维细胞生长因子-2和血管内皮细胞生长因子对牙周膜细胞和可溶性生长因子均有影响。固定在组织培养板上的生长因子的准确定量是不可能的。因此，在本研究中，很难比较固定化和可溶性生长因子的效果。然而，在应用生长因子进行牙周组织再生的情况下，固定化生长因子的利用可能会取代可溶生长因子的缓释系统。为了评价固定化生长因子S在牙周组织中的效果，还需要进一步的研究。

Matricrine effects of basic fibroblast growth factor (FGF-2) on periodontal ligament cells were my estigated using FGF-2 bound to immobilized heparin. In the presence of fetal bovine serum, FGF-2 bound to heparin increased proliferation of periodontal ligament cells and inhibited alkaline phosphatase activity and type I collagen production. Immobilized FGF-2 and FCS directly on tissue culture plates also up-regulated DNA synthesis and down-regulated alkaline phosphatase activity in periodontal ligament cells. These results indicated that immobilized growth factors such as FGF-2 and ECS could affect on periodontal ligament cells as well as those soluble growth factors. The accurate quantification of growth factor immobilized on tissue culture plates was impossible. Thus, it was hard to compare the effectiveness of each growth factor between immobilized form and soluble form in this study. However, in the case of application of growth factor for periodontal tissue regeneration, the utilization of, immobilized growth factor may take the place of slow delivery system of soluble growth factors. Further investigation will be needed in order to evaluate the effectiveness of immobilized growth factor s in periodontal tissues.