Expression of targets for chemotherapy in primacy and metastatic cancer

首先，通过RTPCR和免疫组化分析检测肿瘤化疗靶分子如药物转运蛋白（ATP 7B、MRP 2）、DNA修复分子（CPG、XPF）和FU靶分子如TP、DPD、TS的表达水平。TP和TS在原发灶和转移灶中的表达水平不同。接下来，使用TS或TP的转染子，在动物模型中检查这些分子的表达水平的增强。不幸的是，没有获得这些分子的增强。

First, the expression levels of the target molecules for cancer chemotherapy such as drug transporters (ATP7B, MRP,2), DNA repair molecules (CPG, XPF) and the targets for FU such as TP, DPD, TS were examined by RTPCR and immunohistochemical analysis. The exprnssian levels of TP and TS were different between primary and metastatic lesions among the examined molecules. Next, using the transfectants of TS or TP, the enhancement of the expression levels of these molecules were examined in animal models.Unfortunately, the enhancement of these molecules were not obtained.