Biological and genetic analysis of Vibrio vulnificus in the slime of tidal flats, seawater, and fishes colleted along Ariake Sea, Japan

为了研究日本有宅海创伤弧菌的季节变化，我们尝试从有宅海沿岸三个港口的潮滩、海水和鱼类的粘液中分离创伤弧菌。从2001年1月至12月，每月采集两次样本。创伤弧菌全年均有分离，但在6~10月的暖季分离率较高。温暖季节的菌株能够在含有0.5%氯化钠的培养基上生长，而在其余季节分离的菌株不能生长。此外，温暖季节的分离株比其余季节的分离株表现出更强的溶血活性。这些结果表明，在温暖季节分离的创伤弧菌比在其余季节分离的创伤弧菌更具活力。利用50微米硫脲的脉冲场凝胶电泳法成功地进行了遗传分析。然而，在所研究的菌株中没有检测到相同的带型。

To investigate the seasonal change of Vibrio vulnificus in Ariake Sea, Japan, we attempted to isolate V.vulnificus from the slime of tidal flats, seawater, and fishes obtained from three harbors along Ariake Sea. The samples were collected twice a month from January to December,2001. Also, we determined the biological and genetic characteristics of the individual isolates.V.vulnificus were isolated throughout the year, but the isolation ratios were higher during the warmer season from June to October. The isolates in the warmer season were able to grow in culture media containing 0.5% NaCl, whereas those isolated in the remaining seasons could not. Moreover, the isolates in the warmer season showed greater hemolytic activity than those isolated in the remaining seasons. Those results suggest that V.vulnificus isolated in the warmer seasons are more vigorous in nature than those isolated in the remaining seasons.Genetic analysis by pulsed-field gel electrophoresis using 50microM thiourea was successfully achieved. However, no identical band pattern was detected among strains studied.