Characteristics of the alloplasmic lines of eggplant and application of the lines for eggplant breeding

本研究的目的是将3个茄子异质系与茄子野生种S.anguivi、S.kurzii和S.Violaceum的细胞质特性相结合，应用于茄子育种。研究结果如下：1.育性调查表明，3个异质系均表现出稳定的雄性不育和较高的结实率。2.塑料大棚内3个异质系的生长、产量和抗寒性与各自的亲本茄子品系无显著差异。3.3个异质品系的形态与各自的亲本茄子品系没有显著差异。4.线粒体DNA中srRNAV7和nad7 3-4区的PCR-RFLP分析可用于细胞质鉴定。5.简单序列重复序列(ISSR)分析可作为茄子和野生茄子属植物的遗传标记。6.新阐明了以南方茄子为细胞质的异质茄子表现雄性不育的可能性。

The purpose of this study is to apply the characteristics of the three alloplasmic lines of eggplant with the cytoplasm of the wild Solanum species, S.anguivi, S.kurzii and S.violaceum, for eggplant breeding. Summary of research results are shown as follows.1.Invetstigation of fertility clarified that all the three alloplasmic lines showed stable male sterility and high seed fertility.2.Growth, yield and cold resistance of the three alloplasmic lines in a plastic house did not show the significant difference with those of the respective parental eggplant lines.3.Morphology of the three alloplasmic lines did not show the significant difference with those of the respective parental eggplant lines, except for male organs.4.PCR-RFLP analysis in mitochondrial DNA of the regions srRNA V7 and nad7 3-4 was clarified to be useful for cytoplasm identification.5.Inter-simple sequence repeat (ISSR) analysis was clarified to be useful as genetic markers in eggplant and the wild Solanum species.6.It newly clarified the possibility that the alloplasmic eggplant with the cytoplasm of S.virginianum showed male sterility.