Cardiolipin functions in fish polyunsaturated fatty acid metabolism through plasma lipoproteins

采用纤维镜将放射性二十二碳六烯酸（DHA）注入鲤鱼肠内，并将其掺入磷脂中。在肝胰脏中立即检测到DHA。注射后血浆脂蛋白中检出放射性二十二碳六烯酸，但仅检出少量放射性二十碳五烯酸（EPA）。DHA能被白细胞心磷脂吸收，EPA和花生四烯酸不能被鲤鱼心磷脂吸收。本研究利用放射性多不饱和脂肪酸8PUFA9澄清了鲤鱼血浆中胆固醇酯转移蛋白（CETP）和磷脂转移蛋白（PLTP）的活性。鱼的血浆脂蛋白中未检测到心磷脂，但在血细胞特别是白细胞中检测到心磷脂，并将DHA掺入心磷脂中。提示心磷脂是鱼血细胞中DHA功能的重要组成部分。

Radioactive docosahexaenoic acid (DHA) was injected using with the fiberscope into the carp intestine and the fatty acid was incorporated into phospholipids. The DHA was immediately detected in the hepatopancreas.Radioactive docosahexaenoic acid was detected in plasma lipoprotein after injection but radioactive eicosapentaenoic acid (EPA) was detected only small amounts. And DHA was incorporated into leukocyte cardiolipin but EPA and arachidonic acid were not incorporated into carp cardiolipin.In this research the activities of cholesterol ester transfer protein (CETP) and phospholipid transfer protein (PLTP) were clarified in carp plasma using the radioactive polyunsaturated fatty acid 8PUFA9. Cardiolipin was not detected in fish plasma lipoprotein, but in blood cells especially leukocytes cardiolipin was detected and DHA was incorporated into cardiolipin. It is suggested that cardiolipin was important component for the function of DHA in the fish blood cells.