Investigation of beneficial effect of acupuncture treatment on wound healing in experimental model.

本研究通过动物实验模型，从病理和生化两个方面探讨了针刺治疗对创面愈合的有利作用。由于小鼠自然愈合快，建立小鼠实验模型比较困难。术后缝合伤口的严重程度：我们从宏观上估计了严重程度。结论：1.在这种模型和方法中，我们无法发现针刺治疗的有效性。组织学：针刺治疗组大鼠表皮、真皮细胞数明显减少。针刺组表皮和真皮厚度也受到抑制。组织中mRNA水平(RT-PCR法)：针刺组抑制炎性细胞因子TNF-α、IL-1b、IL-6的表达水平。组织酶表达水平：两组大鼠组织转谷氨酰胺酶(75 KDa)表达水平无差异。血清肿瘤坏死因子-α水平：升高幅度不大。

In the present study, we investigated beneficial effect of acupuncture treatment on wound healing by pathological and biochemical methods in experimental model. It was hard to establish the mouse experimental model, because of their rapid natural healing.1. degree of severity in postoperative sutura wound :We estimated the degree of severity macroscopically. We can not find the effectiveness of acupuncture treatment in this model and methods.2. histology :The cell number of infiltrating cell to epidermides and corium were suppressed in acupuncture-treated group. The thickness of epidermides and corium were also suppressed in acupuncture-treated group.3. mRNA level in tissue (RT-PCR method) :The expression levels of inflammatory cytokine, TNF-a, IL-1b and IL-6, were suppressed in acupuncture-treated group.4. expression level of enzyme in tissue :There was no difference in expression level of tissue transglutaminase (75 kDa) two groups.5. serum TNF-α level :It increased very little.