Methodological establishment for vital observation of tumor microcirculation and development of cancer therapy based on the characteristics of tumor microvessl

肺转移是影响原发性肺癌术后预后的重要因素之一。在本研究中，我们计划通过对肿瘤微循环的生命观察研究，开发基于肺转移瘤微血管特征的癌症新疗法。2004年，我们建立了东流大鼠佐藤肺癌肺转移制备方法、荧光显微镜肺表面肿瘤微循环动态观察系统、软X线微血管造影肺转移肿瘤供血动脉动态观察系统。2005年，我们用荧光显微镜和软X线显微血管摄影研究了肿瘤微血管的特征。2006年，我们研究了ZD6126对肿瘤微血管的影响，化疗免疫治疗（0K432联合CDDP）对抗肿瘤淋巴细胞的影响，缺氧对肺转移供血动脉的影响。（结果）1)建立佐藤肺癌肺转移模型。2)阐明肿瘤微血管的特点。3) ZD6126通过破坏肿瘤微血管对肿瘤造成损伤。4)与CDDP结合的OK432向肿瘤微血管聚集抗肿瘤淋巴细胞。5)缺氧引起肿瘤供血动脉血管收缩。这一结果表明肿瘤血管靶向治疗是可行的。

Lung metastasis is one of the worst factors for postoperative prognosis in primary lung cancer. In this study, we planed to develop new cancer therapy based on characteristics of tumor micro-vessel of lung metastasis through studies of vital observation of tumor microcirculation.During 2004, we established the method for making pulmonary metastasis of SATO lung cancer in Donryu rat, the system for vital observation of tumor microcirculation on surface of the lung by fluorescent microscopy, and the system for vital observation of tumor feeding arteries for lung metastasis by soft X ray micro-angiography.During 2005, we studied the characteristics of tumor micro-vessel by fluorescent microscopy and soft X ray micro-angiography.During 2006, we studied the effect of ZD6126 to tumor micro-vessel, the effect of chemo-immunotherapy (0K432 with CDDP) to anti-tumor lymphocytes, and the effect of hypoxia to feeding arteries of lung metastasis.(RESULTS)1) We established the model of lung metastasis of SATO lung cancer.2) We elucidated the characteristics of tumor micro-vessels.3) ZD6126 injure the tumor through the destruction of tumor micro-vessels.4) OK432 with CDDP accumulate anti-tumor lymphocytes to tumor micro-vessels.5) Hypoxia evokes vasoconstriction of tumor feeding arteries.This result indicates the ability of tumor vascular targeting therapy.