An experiment of making microaspiration pneumonia model mouse with applications of Micro-Fabrication technology

本文介绍了“应用微制造技术制作微吸入性肺炎模型小鼠实验”的研究结果。对8例与显微抽吸有关的尸检病例进行了显微分析。免疫组织化学显示组成胸膜的肺泡上皮细胞(1型)和梭形细胞呈阳性细胞凋亡。在支气管周围的支气管上皮细胞和平滑肌细胞中也发现了阳性结果。为了用实验病理学来证实这一结果，我们对老年大鼠患龋组(实验组)和老年大鼠组(对照组)进行了人工抽吸。与对照组相比，实验组可见大量中性粒细胞浸润和肺水肿。病理检查发现，实验组大鼠肺泡内可见循环系统紊乱和纤维化。支气管上皮细胞抗单链DNA阳性结果提示，细胞的剥离和/或脱落是由细胞凋亡加速所致，这种细胞凋亡的加速现象与尸检结果相似。动物实验证实，衰老和慢性炎症加速了支气管上皮细胞的凋亡，与循环系统障碍相比，对照组出现了更多的衰老现象，肺泡细胞脆性改变。慢性炎症引起的支气管上皮细胞脱落是吸入性肺炎更重要的因素，而不是年龄变化，如脆性变化。

This report is described about the results of the research titled "An experiment of making microaspiration pneumonia model mouse with applications of Micro-Fabrication technology". Eight autopsy cases that related microaspiration were analyzed microscopically. Positive findings of apoptosis were found in bronchoalveolar epithelial cells (type 1) and spindle cells which composing pleura, immunohistochemically. Positive findings were also found in bronchoepithelial cells and smooth muscle cells surrounding the bronchus。 This result suggested that acceleration of apoptosis found in these cells is one of important factor for microaspiration.For the purpose of confirmation for these findings using experimental pathology, artificial aspiration had done for senior rats group suffering caries (Experimental group) and senior rat group(Control group). Much infiltration of neutrocytes and pulmonary edema in experimental group were seen significantly compare to control group. In bronchoalveolar of experimental group, circulatory system disorder and fibrosis were found, pathologically. And much positive findings of anti ssDNA on bronchoepithelial cells was suggested that peeling off and/or falling off of cells caused by acceleration of apoptosiso This acceleration of apoptosis seen in bronchoalveolar cells was similar to the findings of autopsy cases. Acceleration of apoptosis seen in bronchoepithelial cells caused by aging and chronic inflammation was confirmed using animal experiment.On the contrary, there were much aging phenomenon were seen in control group compare to circulation system disorder; fragile change of bronchoalveolar cells. Falling off of bronchoepithelial cells caused by chronic inflammation was more important factor for aspiration pneumonia than aging change, such as fragile change.