New bone formation using porous apatites with cultured bone marrow cells in back of rats

我们开发了烧结碳酸磷灰石（CAP）多孔块，并将其与从年轻成年大鼠获得的骨髓细胞相结合。将这种混合材料植入大鼠背部。骨髓内细胞杂交体作为一种诱导性骨替代材料，其作为人工骨填充材料的应用已得到深入研究。特别是磷酸钙已被研究。本研究的目的是比较四种骨填充材料的成骨效果。所用材料为β-磷酸三钙、碳酸磷灰石、羟基磷灰石和聚苯乙烯作为对照。取青年大鼠股骨骨髓基质细胞，分别培养7天和14天。然后用真实的时间RT-PCR方法检测细胞mRNA表达量。检测runx 2、碱性磷酸酶、骨钙素、骨桥蛋白、骨连接素、骨唾液蛋白、核心蛋白聚糖和1型胶原的基因表达，并进行统计学分析（Scheffe，p<0.05）。结果表明，β-TCP、CAP和HAP的mRNA表达量均高于聚苯乙烯（对照）。而β -TCP、CAP、HAP之间无显著性差异。最后，应用13 -TOP、CAP和HAP作为骨填充材料，证明了其有效性。然而，关于骨形成，所研究的材料的能力没有不同。

We have developed porous blocks of sintered carbonate apatite (CAP) and combined them with bone marrow cells obtained from young adult rats. This hybrid material was implanted into the back of rats. Within 4marrow-cell hybrid is useful as an osteoinductive bone substitute.the use of artificial bone filling materials has been deeply studied. In particular the Calcium phosphate has been investigated. The purpose of this study was to compare the effect of bone formation using four kinds of bone filling materials. The materials employed were, β-tricalcium phosphate, carbonate apatite, hydroxyapatite and polystyrene as control. Bone marrow stromal cells of femora from young adult rats were cultured for 7 and 14 days respectively. After that, the quantity of mRNA expression of the cells cultured was measured by Real Time RT-PCR method. The gene expression of runx2, alkaline posphatase, osteocalcin, osteopontin, osteonectin, bone sialoprotein, decorin and type1 collagen was measured and statistically analyzed (Scheffe, p<0.05). The results showed that the quantity of mRNA expression in β-TCP, CAP and HAP was higher than the polystyrene (control). However, there was no significant difference amongst β -TCP, CAP and HAP. Finally, the application of 13 -TOP, CAP and HAP as bone filling materials, demonstrated effectiveness. Nevertheless, concerning the bone formation, the capacities of the materials studied were not different.