Lethal effects of exposure to environmental cigarette smoke on Drosophila larvae and its oxidative damage transmitted to the next generation

流行病学证据表明，吸烟对我们的健康有害。许多调查都记录了主流香烟烟雾造成的损害。本文以果蝇为研究对象，研究了环境香烟烟雾（ECS）引起的生物效应。当3龄幼虫暴露于ECS时，与野生菌株相比，无尿酸突变菌株的羽化率显著降低。由于尿酸盐被认为是果蝇体内的一种抗氧化剂，香烟似乎会引起幼虫的氧化损伤。为了评估ECS对后代发育的影响，我们观察了暴露于ECS或未暴露于ECS的幼虫中雌雄交配产生的卵的存活率。雄性和雌性同时暴露的无尿酸菌株的存活率最低。这些结果表明，ECS对三龄幼虫的损害不仅影响其自身的生存能力，而且影响其下一代的生存能力。

Epidemiological evidence indicates that cigarette smoke is harmful for our health. Many investigations have documented damage induced by mainstream cigarette smoke. We examined the biological effects induced by environmental cigarette smoke (ECS) using Drosophila. When 3rd instar larvae were exposed to ECS, the eclosion-rate of a urate-null mutant strain decreased significantly compared to that of a wild strain. As urate is considered to function as an antioxidant in Drosophila, cigarette smoke appears to induce oxidative damage in larvae. To estimate the effect of ECS on offspring development, we observed the viability of flies emerging from eggs that were produced by matings between males and females eclosed from larvae exposed or not exposed to ECS. Pairs of the urate-null strain in which both males and females were exposed showed the lowest viability. These results suggest that third instar larvae were damaged by ECS affecting not only their own viability but also that of the next generation.