Local neuronal circuit in the motor cortical areas

我们开发了一种研究运动皮质区域局部回路的工具，采用“从一个到组”的策略，在局部回路上得到了以下结果：(1)分析了gaba能皮质中间神经元对皮质脊髓投射神经元（csn）的局部输出，发现各中间神经元组对csn的神经支配存在差异。(2)在小白蛋白（PV）启动子的特异性控制下，培养了表达树突膜GFP的BAC转基因小鼠和大鼠，并检测了表达PV的中间神经元的兴奋性和抑制性输入。(3)我们开发了能够逆行标记皮质神经元的腺病毒载体，并研究了锥体神经元与皮质丘脑投射神经元的局部连接。

We developed a tool for the study of the local circuit of the motor cortical areas, and obtained the following results on the local circuit using the "From one to group" strategy:(1)The local output of GABAergic cortical interneurons to corticospinal projection neurons (CSNs) was analysed, and found that each interneuron group differentially innervated CSNs.(2)We developed BAC transgenic mice and rats expressing dendritic membrane targeted GFP under the specific control of parvalbumin (PV) promoter, and examined the excitatory and inhibitory inputs to PV-expressing interneurons.(3) We developed adenoviral vector that enable Golgi-stain-like retrograde labeling of cortical neurons, and studied the local connections of pyramidal neurons to corticothalamic projection neurons.