Impact of Arabidopsis nuclear lamina disassembly on chromatin under abiotic stresses

核纤层（NL）是由核纤层蛋白和核纤层蛋白相关的核膜蛋白形成的复杂网络，其支撑核以维持结构完整性。在拟南芥中，核基质组成蛋白（NMCP）是NL的重要组成部分，是维持核结构完整性和特定核周染色质锚定所必需的。到目前为止，很少有人知道有关的动态NL-染色质相互作用在植物中。在我们最近的工作中，我们发现在拟南芥中，核周锚定的染色质区域在热胁迫下脱离核膜。此外，我们还发现各种非生物胁迫都会引起拟南芥NL的解体。这一发现促使我们实现这个项目，以更好地了解拟南芥中动态NL-染色质相互作用的性质。该项目分为三个部分。首先，我们将在各种压力条件下分析NL-染色质相互作用和核纤层相关结构域的空间分布。在第二部分中，我们计划采用蛋白质组学方法来探索核层蛋白的翻译后修饰，并旨在确定其对核层组装的功能意义。在第三部分中，通过生成高分辨率Hi-C图谱，我们的目标是揭示核纤层蛋白在应激适应过程中调节三维基因组组织的结构作用。

The nuclear lamina (NL) is a complex network formed with nuclear lamins and lamin-associated nuclear membrane proteins, which scaffold the nucleus to maintain structural integrity. In Arabidopsis thaliana, Nuclear Matrix Constituent Proteins (NMCPs) are essential components of the NL and are required for maintaining the structural integrity of the nucleus and specific perinuclear chromatin anchoring. So far, little has been known concerning the dynamics of NL-chromatin interactions in plants. In our recent work, we found that in Arabidopsis, perinuclear anchored chromatin regions detached from the nuclear envelope under heat stress. Besides, we found that various abiotic stresses caused the Arabidopsis NL to disassemble. This finding prompted us to realise this project to gain a better understanding of the nature of the dynamic NL-chromatin interactions in Arabidopsis. There are three parts in the project. Firstly, we will profile NL-chromatin interactions and the spatial distribution of lamina-associated domains under various stress conditions. In the second part, we plan to employ proteomic approaches to explore post-translational modifications on nuclear lamin proteins and aim to establish their functional implication to the nuclear lamina assembly. In the third part, by generating high-resolution Hi-C maps, we aim to reveal the structural role of nuclear lamins in modulating three-dimensional genome organization during stress adaptation.