Architecture and functional significance of nuclear laminae of different molecular composition

核层是核骨架的主要组成部分。其结构具有细胞类型特异性，其分子组成受发育调控。然而，其分子结构差异的功能意义尚不清楚。我们将在两栖动物卵母细胞中使用特殊层粘连蛋白或层粘连蛋白突变体的异位表达来实验性地改变体内层粘连的组成。这种策略将使我们能够分析层状结构内部的分子相互作用，以及与核膜和核内部的其他成分的相互作用。这些实验旨在为膜层在维持核膜和细胞核的完整性和机械强度方面的作用以及在建立/维持核孔复合物的空间格局方面的潜在作用提供实验支持。这些分析将作为了解其他生物系统中更复杂的层状结构的功能的第一步，由于技术原因，这些结构不能或很难进行详细的分子和结构分析。

The nuclear lamina is a main constituent of the nuclear skeleton. Its structure is cell type specific and its molecular composition is developmentally regulated. However, the functional significance of the differences in its molecular architecture is not clear. We will use ectopic expression of particular lamin proteins or lamin mutants in amphibian oocytes to experimentally alter the composition of the lamina in vivo. This strategy will allow us to analyze the molecular interactions within the lamina structure itself as well as with other components of the nuclear envelope and the nuclear interior. These experiments are aimed to give experimental support for the role of the lamina in maintaining the integrity and mechanical strength of the nuclear envelope and the nucleus and its potential role in establishing/maintaining the spatial pattern of the nuclear pore complexes. The analyses will serve as a first step to understand the function of more complex lamina structures in other biological systems, that are - for technical reasons - not or only hardly accessible to a detailed molecular and structural analysis.