Investigations for inhibition of arterial restenosis after metallic stent placement.

1)本实验采用曲尼司特对家兔动脉再狭窄的抑制作用。所有家兔均在腹主动脉置入金属支架。支架置入4周后全部处死，取主动脉，制作主动脉标本，显微镜观察。在支架放置前一周和放置后四周，10只兔子中有5只口服曲尼司特，另一只不口服。显微镜下，所有标本均显示内膜增生。曲尼司特组与对照组相比，增生面积明显减少。2)金属支架上的壳聚糖涂层：将壳聚糖（1g）溶于醋酸（1N, 100ml）中。将金属支架放入溶液中取出，放入NaOH（1N, 100ml）中。虽然金属支架表面附着了一些壳聚糖颗粒，但涂层不均匀，不完整。进一步调查是必要的。3)抗癌药物对血管内皮功能的影响。本实验检测法莫比星（EPIR）、丝裂霉素（MMC）和氟尿嘧啶（5-FU）。取大鼠胸主动脉，置于Krebs溶液中。制作螺旋条，评价其对乙酰胆碱（Ach）和硝普钠（SNP）的松弛反应。EPIR影响对Ach的松弛反应，但对SNP没有影响。MMC和5-FU不影响对Ach和SNP的松弛反应。这些结果表明EPIR损害内皮功能。

1) Effects of tranirast on inhibition of arterial restenosisTen rabbits were used for this experiment. Metallic stents were placed in the abdominal aorta in all rabbits. Four weeks after stent placement, all of them were sacrificed and the aortas were taken, and aortic specimen were made and microscopic obserbation was performed. For one week before and four weeks after the stent placemet, tranilast was orally treated for five of ten rabbits, and not for the other. Microscopically, all the specimen revealed intimal hyperplasia. The area of hyperplasia significantly decreased in the tranilast group than it in control.2) Chitosan coating on metallic stentChitosan(1g) was solved in acetic acid(1N, 100ml). The metallic stent was placed into the solution and taken, and it was placed into the NaOH(1N, 100ml). Although some chitosan particles were attached on the surface of metallic stent, the coating seemed uneven and imcomplete. Further investigation would be necessary.3) Effects of anticancer drugs on the vascular endotherial function.Farmorbicin(EPIR), mitomycine(MMC), and fluorouracil(5-FU) were examined for this experiment. The rat thoracic aorta was removed and placed in the Krebs solution. The helical strips were made and the relaxation response to acetylcholine(Ach) and sodium nitroprusside (SNP) were evaluated. EPIR affected the relaxation response to Ach but did not it to SNP. MMC and 5-FU did not affect the relaxation response to Ach and SNP. These results reveal EPIR impairs endotherial function.