Reaction mechanisms of various growth regulators and differentiation regulators and their clinical applications

F. Suzuki证明，在培养的兔软骨细胞上，胎牛软骨含有生长激素样因子和内皮细胞生长抑制剂。他认为细胞对生长激素样因子的反应高度依赖于软骨细胞的密度。Yamane （T. Satoh）从人胚胎成纤维细胞无血清培养基中纯化成纤维细胞来源的内皮细胞生长因子（f-ECGF）。f-ECGF是一种酸性糖蛋白，其等电点在pH范围4.5-6，Mr约为。30 kD.K。Shizume发现人垂体前叶组织含有Mr为15 kD的耐热和耐酸tgf样丝裂原。该因子不与EGF竞争受体结合，也不需要EGF来形成集落活性。Takaku发现肾细胞癌和肝细胞癌在每个患者中都产生明显的红细胞生成。从两种肿瘤细胞的条件培养基中纯化了促红细胞生成素样活性。Takeda发现，与牛血清白蛋白氨基酸残基115-143和144-184（185）相对应的一种双链多肽增强了胰岛素对离体大鼠脂肪细胞葡萄糖代谢的影响。Ichihara发现，大鼠血小板不仅含有一种生长因子（肝细胞生长因子，HGF），还含有至少两种影响成年大鼠肝细胞的生长抑制剂（血小板衍生生长抑制剂- α， pdgi - α和pdgi - β）。Nishikawa从大鼠罗丹明纤维肉瘤组织中纯化了肿瘤生长刺激活性（TGSA）。他认为TGSA是一种由肿瘤细胞产生的新型强效生长因子，能够支持肿瘤细胞自身的生长。Kishimoto从培养的TCL-Nal细胞上清液中纯化人b细胞分化因子（BCDF）至均匀性。纯化BCDF诱导eb病毒转化细胞系分泌Ig。

F. Suzuki demonstrated that fetal bovine cartilage contains both a somatomedin-like factor on cultured rabbit chondrocytes and an endothelial cell growth inhibitor. He suggested that the cellular response to somatomedin-like factor was highly dependent on the density of chondrocytes.I. Yamane (T. Satoh) purified fibroblast-derived endothelial cell growth factor (f-ECGF) from serum-free medium of human embryo fibroblasts. f-ECGF is an acidic-glycoprotein with an isoelectric point in the pH range 4.5-6, and Mr approx. 30 kD.K. Shizume found that human anterior pituitary tissues contain heat- and acid-stable TGF-like mitogen(s) with Mr 15 kD. This factor did not compete with EGF for receptor binding and did not require EGF for colony forming activity.F. Takaku found that a renal cell carcinoma and a hepatocellular carcinoma produce a marked erythropoiesis in each patient. The erythropoietin-like activities were purified from the conditioned media from both tumor cells.Y. Takeda found that a two-chain polypeptide, which corresponds to amino acid residues 115-143 and 144-184 (185) of bovine serum albumin, potentiated the effects of insulin on glucose metabolism in isolated rat adipocytes.A. Ichihara found that rat platelets contain not only a growth factor (Hepatocyte growth factor; HGF), but also at least two types of growth inhibitor (Platele-derived growth inhibitor-alpha; PDGI-alpha, and PDGI-beta) that affect adult rat hepatocytes.K. Nishikawa purified tumor growth stimulating activity (TGSA) from the rat Rhodamine fibrosarcoma tissue. He suggested that TGSA, a new class of potent growth factor produced by tumor cells, supports their own growth.T. Kishimoto purified human B-cell differentiation factor (BCDF) to homogeneity from cultured supernatants of TCL-Nal cells. Purified BCDF induced Ig secretion in Epstein-Barr virus-transformed cell lines.