Study on Physiological Function of Oxygenated Sterols in Blood

本研究探讨了氧合甾醇对血液成分（如红细胞和血小板）的各种生物学效应与动脉粥样硬化和心脏病等疾病的关系，结果如下：(1)在培养的牛颈动脉内皮细胞中发现谷甾醇和焦糖甾醇能产生纤溶酶原激活剂。(2)发现一些氧合甾醇可引起血浆中血小板聚集的快速调节。（22R）-22-羟基胆固醇：通过与细胞膜结合，特异性地进行血小板和红细胞的溶解。(3)合成了侧链上带有生碳取代基的胆固醇类似物，并在模型体系中研究了它们的光化学反应。(4)对真菌感染的家蚕（Bombyx cum Botryte）尸体氧合甾醇进行了分析。(5) 15-氧合甾醇被发现是二氢羊毛甾醇转化为胆固醇的有效抑制剂。(6)研究了与本课题相关的具有生物活性的天然甾醇和萜类化合物，并确定了它们的结构。(7)分析高血压、动脉粥样硬化患者血清中氧合固醇的含量。

This study deals with various biological effects of oxygenated sterols on blood components such as erythrocytes and platelets in relation with diseases like atherosclerosis and heart disease, and resulted in the following findings.(1) Sitosterol and fucosterol were found to produce plasminogen activator in cultured endothelial cells from bovine carotod artery.(2) Some of the oxygenated sterols found to cause rapid modulation of platelet aggregation in plasma. (22R)-22-Hydroxycholesterol found to specifically undertakes lysis of platelets and erythrocytes through its incorporation into membrane.(3) Cholesterol analogues with a carbene-generating substituent on the side chain were synthesized and their photochemical reactions were investigated in a model system.(4) Oxygenated sterols of fungus-infected silkworm carcass, Bombyx cum Botryte were analyzed.(5) 15-Oxygenated sterols were found to be a potent inhibitor of enzymatic conversion of dihydrolanosterol into cholesterol.(6) Natural sterols and terpenoids with biological activities related in this project were investigated and their structures were determined.(7) Serum sterols from patients (hypertension, atherosclerosis) were analyzed for oxygenated sterols.