Experimental Studies on Ecotypic Differentiation of Plantago asiatica L. in Japan islands.

亚洲车前在日本岛屿上大致可分为两个不同的生态型。一种是在低温短日照下进入休眠状态的夏绿型(Type-I)，另一种是在相同条件下继续生长的常青型(Type-II)。比较了日本岛屿不同地点采集的9种不同种子幼苗的同工酶图谱。因此，我们找不到任何标记同工酶用于两种类型车前的分类。本项目对不同类型车前幼苗的亚种和陆生部分的物质分配进行了比较研究。在第I类植物中，陆生部分的可溶性蛋白质在倒生过程中大量迁移并积累在陆生部分。

Plantago asiatica L. in Japan islands can be roughly classified into two different ecotypes. One is a summer green type (Type-I) which becomes to dormant state under photoperiod of short-day at low temperature, and the other is a ever green type (Type-II) which continues to grow under the same conditions.We compared the patterns of the zymograms of 9 different isozymes of seedling of seeds collected from the various sites in Japan islands. As a results, we could not find any marker isozyme to use for the classification of two types of Plantago asiatica.Comparative studies on materials partitioning between sub- and terresterenean parts of seedling of different types of Plantago asiatica were performed in this project. In Type-I plants, a considerable amounts of soluble proteins in terresterenean part moved to and accumulated in subterresterenean part during the progress of dormancy.Conditions for the isolation of protoplasts from leaves of Plantago asiatica were also investigated.