Molecular mechanism of initiation of DNA replication

许多研究者合作研究了各种基因组DNA复制起始的机制。质粒DNA复制起始的调节已被分析，重点是起始蛋白和每个质粒的起始区域的靶DNA序列之间的相互作用。质粒pSC 101、Co 1 E1、Co 1 E2、Co 1 E3、dv和E. colioriC质粒进行了研究。E. coli oriC进行了体内和体外分析。B的基因组织。subtilis复制起点与E.大肠杆菌基因组已经被揭示。已经确定了各种基因组复制起点的引发位点（从引物RNA到DNA的过渡位点）。R6 K和E. coli基因组的克隆。F质粒基因的结构和功能的分配所必需的已澄清。已经从哺乳动物细胞中克隆了自主复制序列，并且已经显示了c-myc产物在促进复制起始中的可能功能。胸苷酸合成酶基因的结构已被确定，转录调控已被研究。花椰菜花叶病毒（CaMV）基因组编码的逆转录酶已被研究，RNA作为CaMV DNA复制的中间产物参与其中。

Mechanism of initiation of DNA replication of various genomes has been studid co-operatively by many investigators. Regulation of the initiation of plasmid DNA replication has been analyzed in emphasis on the interaction between initiator proteins and target DNA sequence at the origin region of each plasmid. Plasmids R6K, F, pSC101, Co1E1, Co1E2, Co1E3, dv and E. coli oriC plasmid have been investigated. Transcription regulation in E. coli oriC has been analyzed in vivo and in vitro. Gene organization of B. subtilis replication origin has been analyzed and high similarities to E. coli genome have been revealed. Priming sites (transition sites from primer RNA to DNA) at replication origins of various genomes have been determined. fermination loci of DNA replication of R6K and E. coli genome have been cloned. Structure and function of the F plasmid genes essential for partitioning has been clarified. Autonomously replicating sequences have been cloned from mammalian cells and possible function of c-myc product in promotion of initiation of replication has been shown. The structure of thymidylate synthase gene has been determined and transcription regulation has been studied. Reverse transcriptase encoded by cauliflower mosaic virus (CaMV) genome has been investigated and involvement of RNA as an intermediate of CaMV DNA replication has been shown.