Chemical Studies on the Mechanisms of Iron Absorption in Chattonella spp.

濑户濑户内海Harima Nada海水中的铁含量远低于Chattonella spp.他们的增长导致本作者假设存在的积极吸收机制的铁Chatonella属。从海马纳达赤潮中采集的养殖古生和海水古生两种藻样，用80%乙醇提取，提取液分别用柱层析和薄层层析进行处理。在C.antiqua和C. marina中证实了铁螯合物质的存在。利用核磁共振和色谱技术确定了海洋卡盾藻的螯合物质之一为去铁胺B，并在卡盾藻的外膜周围发现了糖萼层，并假定该层与所含的酸性多糖一起参与铁的吸收。用<Na_2><CO_3>DEAE纤维素柱，以NaCl（0.25 - 2.0M，pH 2和pH 12）为梯度洗脱剂，从古槐细胞中提取酸性多糖，并进行分级。磺化多糖组分在17个葡萄糖分子中结合1个铁原子。查顿氏菌重金属含量的研究。利用日立300型等离子体光谱仪分析了浮游植物对铁离子的吸收，并初步探讨了其吸收机制。可溶性或颗粒状的铁与酸性多糖结合吸附在糖萼层上，然后转移到细胞分泌的铁螯合物质（铁载体）中，转运到细胞内用于生化目的。

The facts that iron concentrations in sea water of Harima Nada, Seto Inland Sea, are much less than that required by Chattonella spp. for their growth led the present authors to assume the presence of active absorption mechanisms of iron in Chatonella spp. Cutured C.antiqua and C.marina sampled from red tide of Haima Nada were extracted with 80 % alcohol and the extracts were treated with column and thin layer chromatographies. Presence of iron chelating substances were confirmed in both C.antiqua and C.marina. One of the chelating substances of C.marina was ascertained as deferroxamine B by NMR and chromatographic techniques.Glycocalyx layer was also found around the outer membrane of Chattonella spp., and the layer was assumed to participate in iron abosorption with the acidic polysaccharides contained. Acidic polysaccharides were extracted from C.antiqua cells with <Na_2> <CO_3> and fractionated with DEAE cellulose column by stepwise gradient elution using NaCl (0.25 - 2.0 M,pH 2 and pH 12). Sulfonated polysaccharide fraction conjugates 1 atom of iron in 17 molecules of it glucose. Heavy metal contents in Chattonella spp. and other phytoplankton were also analyzed with plasma spectrometer (Hitachi 300).Based on these results, the iron absorption mechanisms are postulated to be as follows. Iron, either soluble or particulate from, is adsorbed on glycocalyx layerin conjugation with acidic polysaccharides, and, then, transferd to iron chelating substances (siderophores) excreted from cell to transport into the cell for the biochemical purposes.